NF-κB调节TNF-α表达减轻大鼠缺血性脑血管病脑组织损伤

发布时间：2018-05-04 12:19

本文选题：缺血性脑血管病 + 脑损伤　；参考：《中国老年学杂志》2017年10期

【摘要】：目的研究缺血性脑血管病(ICVD)核因子(NF)-κB对肿瘤坏死因子(TNF)-α表达的影响及其对大鼠脑组织损伤的作用。方法将48只Wistar大鼠随机分成正常组(12只)、模型组(12只)、假手术组(12只)和吡咯烷二硫基甲酸酯(PDTC)+模型组,每组12只。采用线栓法制成大鼠局部缺血再灌注模型,用Ludmila Belayev 12分评分法评估大鼠神经功能,氯化-2,3,5-三苯基四氮唑(TTC)染色法观察脑梗死体积,免疫组化法检测脑组织NF-κB表达,酶联免疫吸附法(ELISA)测脑组织TNF-α含量。结果模型组大鼠Ludmila Belayev 12得分明显高于正常组与假手术组(P0.05),PDTC+模型组得分显著低于模型组(P0.01);模型组大鼠的脑梗死体积显著多于正常组和假手术组(P0.01),与模型组比较,PDTC+模型组大鼠脑梗死体积显著减少(P0.05);模型组大鼠脑组织NF-κB阳性细胞表达率显著高于正常组和假手术组(P0.05),DPTC+模型组NF-κB表达明显低于模型组(P0.05);模型组大鼠TNF-α的含量显著高于正常组和假手术组(P0.01),PDTC+模型组TNF-α含量明显低于模型组(P0.05)。结论 PDTC抑制NF-κB活性可以减少TNF-α含量,降低TNF-α的表达,所以可通过NF-κB调节TNF-α表达对缺血再灌注脑损伤起到保护作用,可能与其抑制炎症反应作用有关。
[Abstract]:Objective to study the effect of NF-魏 B on the expression of TNF- 伪 and its effect on brain tissue injury in rats with ischemic cerebrovascular disease (ICVD). Methods 48 Wistar rats were randomly divided into normal group (n = 12), model group (n = 12), sham operation group (n = 12) and pyrrolidine dithioformate group (n = 12). The rat model of local ischemia reperfusion was established by the method of thread embolization. The nerve function was evaluated by Ludmila Belayev 12 score method, the infarct volume was observed by chlorination and the expression of NF- 魏 B in brain tissue was detected by immunohistochemical method, and the cerebral infarct volume was observed by using the method of trichlorotriphenyl tetrazolium chloride (TTC), and the expression of NF- 魏 B in brain tissue was detected by immunohistochemistry. The content of TNF- 伪 in brain tissue was measured by enzyme linked immunosorbent assay (Elisa). Results the scores of Ludmila Belayev 12 in the model group were significantly higher than those in the normal group and the sham operation group, and the scores in the model group were significantly lower than those in the model group, and the volume of cerebral infarction in the model group was significantly larger than that in the normal group and the sham operation group (P 0.01), and compared with that in the model group, the cerebral infarction volume in the model group was significantly higher than that in the control group and the sham operation group. The positive expression rate of NF- 魏 B in the model group was significantly higher than that in the normal group and sham operation group. The expression of NF- 魏 B in the model group was significantly lower than that in the model group, and the content of TNF- 伪 in the model group was significantly higher than that in the model group. The content of TNF- 伪 in the normal group and the sham operation group was significantly lower than that in the model group. Conclusion PDTC can reduce the content of TNF- 伪 and decrease the expression of TNF- 伪 by inhibiting the activity of NF- 魏 B, so it may play a protective role in cerebral ischemia-reperfusion injury by regulating the expression of TNF- 伪 through NF- 魏 B, which may be related to the inhibition of inflammation.
【作者单位】：南阳市第三人民医院神经内科;南阳市中心医院病理科;
【分类号】：R743

【相似文献】