血管活性肠肽在MPTP帕金森病模型小鼠的抗氧化应激作用

发布时间：2018-05-06 09:13

本文选题：帕金森病 + 血管活性肠肽　；参考：《神经解剖学杂志》2017年03期

【摘要】：目的:研究血管活性肠肽(VIP)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森病(PD)模型小鼠发挥抗氧化应激和神经保护作用。方法:雄性C57BL/6J小鼠随机分为生理盐水(NS)组、MPTP组和MPTP+VIP组。Elisa法检测纹状体丙二醛(MDA)以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的变化;免疫组织化学法观察中脑黑质纹状体系统酪氨酸羟化酶(TH)、星形胶质细胞特异性标记物胶质细胞纤维酸性蛋白(GFAP)和小胶质细胞标志物离子钙结合蛋白(Iba-1)的表达变化;透射电子显微镜观察中脑黑质多巴胺能神经元的超微结构变化。结果:MPTP组与对照组相比,MDA水平显著增高,SOD和CAT的表达显著降低;给予VIP可显著抑制MDA的水平(P0.01),增强SOD和CAT的表达(P0.05)。与对照组相比,MPTP组小鼠GFAP和Iba-1的表达明显上升,TH表达明显下降;给予VIP可显著降低GFAP和Iba-1的表达(P0.05),而TH表达明显增强。透射电镜观察显示:NS组神经细胞和细胞器结构清晰完整;MPTP组神经细胞核膜内陷,线粒体空泡样变;MPTP+VIP组神经细胞和细胞器结构基本正常。结论:VIP能够抑制MPTP诱导PD小鼠中脑黑质星形胶质细胞和小胶质细胞的活化,对抗氧化应激,发挥神经保护作用。
[Abstract]:Aim: to study the antioxidation and neuroprotective effects of vasoactive intestinal peptide (VIP) on PD model mice induced by 1-methyl-4-phenyl-1-trihydropyridine (MPTP). Methods: male C57BL/6J mice were randomly divided into two groups: MPTP group and MPTP VIP group. The changes of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (catalase) in the striatum were detected by Elisa. The expression of tyrosine hydroxylase (THN), astrocyte-specific marker glial fibrillary acidic protein (glial fibrillary acidic protein) and microglial marker ion-calcium-binding protein (Iba-1) in the substantia nigra striatum were observed by immunohistochemical method. The ultrastructural changes of dopaminergic neurons in the substantia nigra were observed by transmission electron microscope. Results compared with the control group, the expression of sod and CAT was significantly decreased in the control group, and the expression of SOD and CAT was significantly inhibited by VIP, and the expression of SOD and CAT was enhanced by the administration of VIP. Compared with the control group, the expression of GFAP and Iba-1 in MPTP group increased significantly, and the expression of th decreased significantly in mice treated with VIP, and the expression of GFAP and Iba-1 decreased significantly, while the expression of th increased significantly. The results of transmission electron microscope showed that the structure of neurons and organelles in the VIP group was clear and intact. The nuclear membrane of the neurons in the MPTP group was trapped, and the structure of the neuronal and organelle in the MPTP VIP group was basically normal. ConclusionVIP can inhibit the activation of astrocytes and microglia in the substantia nigra of PD mice induced by MPTP, and play a neuroprotective role on antioxidant stress.
【作者单位】：潍坊医学院组织学与胚胎学教研室;潍坊医学院医学研究实验中心;潍坊医学院;潍坊医学院护理学院;
【基金】：山东省医药卫生科技发展计划项目(2015WS0063) 潍坊医学院大学生科技创新基金项目(KX2016006,KX2016011)
【分类号】：R-332;R742.5

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