起源于小鼠诱导性多能干细胞的大脑皮质类器官的建立及其生物学特性

发布时间：2018-05-06 20:22

本文选题：诱导性多能干细胞 + 大脑皮质类器官　；参考：《解剖学报》2017年04期

【摘要】：目的利用小鼠诱导性多能干细胞(iPSCs)建立大脑皮质类器官并分析其生物学特性。方法首先将生长在饲养层细胞上的iPSCs克隆消化成单细胞,悬浮培养使其自发形成拟胚体。随后,对其进行神经上皮分化的诱导。在合适时间将其嵌入Matrigel基质胶做的悬滴中,摇动培养,形成大脑皮质类器官;最后利用免疫荧光、Dio示踪、透射电子显微镜等技术检测大脑皮质类器官的生物学特性。结果大脑皮质类器官有神经上皮、神经元及神经胶质细胞的分化特征;具有简单的片层化结构,如皮质板和分子层,且大脑皮质类器官具有神经再生及修复能力。结论利用iPSCs成功建立了大脑皮质类器官模型,且该模型具有与活体大脑皮质相似的特征,且具有神经再生能力。
[Abstract]:Objective to establish cerebral cortex organs and analyze their biological characteristics by using induced pluripotent stem cells (iPSCs) in mice. Methods iPSCs grown in feeder layer cells was cloned and digested into single cells, which were cultured in suspension culture to form embryoid body spontaneously. Then, the differentiation of neuroepithelium was induced. At the appropriate time, it was embedded in the suspension of Matrigel gel to shake and culture to form cerebral cortical organs. Finally, the biological characteristics of cerebral cortical organs were detected by immunofluorescence tracing and transmission electron microscopy. Results the cerebral cortical organs had the differentiation characteristics of neuroepithelium, neurons and glial cells, and had simple lamellar structures, such as cortical plate and molecular layer, and the cerebral cortical organs had the ability of nerve regeneration and repair. Conclusion the model of cerebral cortical organs was successfully established by using iPSCs. The model has the characteristics similar to that of the in vivo cerebral cortex and has the ability of nerve regeneration.
【作者单位】：河南大学生命科学学院河南大学神经生物学研究所;
【基金】：河南大学省属高校科研基金(0000A40475,0000A40356) 河南省科技攻关计划(122102310495,132102310111)
【分类号】：R741

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