肢体缺血后处理对局灶性脑缺血再灌注大鼠Caspase-3和细胞凋亡的影响

发布时间：2018-05-10 20:39

本文选题：缺血再灌注 + 肢体缺血后处理　；参考：《中国老年学杂志》2017年04期

【摘要】：目的探讨肢体缺血后处理(Lpost)对大鼠局灶性脑缺血再灌注区半胱氨酸蛋白酶(Caspase)-3和细胞凋亡的影响。方法健康雄性SD大鼠36只,随机分为假手术组(Sham组)、缺血再灌注组(I/R组)、肢体缺血后处理组(Lpost组),I/R组、Lpost组均行缺血2 h再灌注24 h局灶性脑缺血再灌注模型。Lpost组再灌注前实施肢体缺血后处理(缺血15 min,灌注15 min)3个循环。各组大鼠神经功能评分,采用四氮唑红(TTC)染色确定脑缺血半暗带位置,TUNEL测定细胞凋亡,免疫组化测定Caspase-3的表达变化。结果 Sham组神经缺损评分为0分;与Lpost组(1.20±0.41)比较,I/R组神经功能缺损较重(2.40±0.51,P0.05);TTC染色证实缺血半暗带位于大脑矢状裂至外侧裂上1/3的皮质组织。与Sham组相比,I/R组、Lpost组缺血半暗带内凋亡细胞数、Caspase-3表达均明显增加(P0.05),且I/R组表达明显增强,高于Lpost组(P0.05)。结论肢体Lpost可减轻脑缺血再灌注损伤诱导的细胞凋亡,缓解神经细胞的坏死,其作用机制可能与减少Caspase-3表达有关。
[Abstract]:Objective to investigate the effects of Lposton on caspase- 3 and apoptosis in focal cerebral ischemia reperfusion area in rats. Methods 36 healthy male Sprague-Dawley rats were used. Randomly divided into sham-operated group, ischemia-reperfusion group, I / R group, limb ischemia post-treatment group, Lpost group, I / R group, Lpost group, 24 h focal cerebral ischemia-reperfusion model. Three circulations (ischemia 15 min, perfusion 15 min). The neuronal function of rats in each group was evaluated by TTC staining. Tunel was used to determine the location of ischemic penumbra and the expression of Caspase-3 was detected by immunohistochemistry. Results the score of nerve defect in Sham group was 0, compared with that in Lpost group (1.20 卤0.41), the nerve function defect in I / R group was more serious than that in Lpost group (2.40 卤0.51) P0.05TTC staining confirmed that the ischemic penumbra was located in the cortical tissue of sagittal fissure to the superior 1 / 3 of the lateral fissure of the brain. Compared with the Sham group, the expression of Caspase-3 in the ischemic penumbra increased significantly in the I / R group, and the expression of Caspase-3 in the I / R group was significantly higher than that in the Lpost group. Conclusion limb Lpost can attenuate apoptosis induced by cerebral ischemia-reperfusion injury and relieve neuronal necrosis. The mechanism may be related to the reduction of Caspase-3 expression.
【作者单位】：桂林医学院附属医院神经内科;
【基金】：广西省自然科学基金(2012jjAA40148) 桂林市科技开发项目(0110119-1-6)
【分类号】：R743.3

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