视神经脊髓炎谱系疾病患者循环滤泡辅助性T细胞和滤泡调节性T细胞的变化

发布时间：2018-05-11 02:24

本文选题：视神经脊髓炎谱系疾病 + 滤泡辅助性T细胞　；参考：《实用医学杂志》2017年03期

【摘要】：目的:探讨循环滤泡辅助性T细胞(cTfh)、循环滤泡调节性T细胞(cTfr)以及cTfh/cTfr比值在视神经脊髓炎谱系疾病(NMOSD)病程中的变化及意义。方法:流式细胞术检测15例复发期、16例缓解期NMOSD患者及18例健康对照者的cTfh、cTfr及外周血B细胞比率;酶联免疫吸附试验检测3组受试者血浆IL-21以及患者血浆抗水通道蛋白4抗体(AQP4-Ab)水平。结果:与缓解期患者和健康人相比,复发期患者cTfh和B细胞比率、cTfh/cTfr比值以及IL-21水平均升高,cTfr比率降低;而缓解期患者与健康人的上述指标相比差异无统计学意义。复发期和缓解期患者AQP4-Ab无明显差异。患者的cTfh比率与B细胞比率、血浆IL-21水平呈正相关,而cTfr比率与IL-21水平之间呈负相关。患者cTfh/cTfr比值与B细胞比率和IL-21之间亦呈正相关,但AQP4-Ab水平与cTfh比率、B细胞比率、cTfh/cTfr比值以及IL-21没有相关性。结论:NMOSD患者cTfh和cTfr比率的变化以及cTfh/cTfr比值失衡可能促进机体内体液免疫应答异常活化,从而参与NMOSD的发病。
[Abstract]:Objective: to investigate the changes and significance of circulating follicular helper T cells (CTFHN), circulating follicular regulatory T cells (CTF) and the ratio of cTfh/cTfr in the course of optic neuromyelitis (cTfh/cTfr). Methods: flow cytometry was used to detect cTfhtfr and the ratio of B lymphocytes in peripheral blood of 15 patients with relapsing stage and 16 patients with remission NMOSD and 18 healthy controls. The levels of plasma IL-21 and plasma anti aquaporin 4 antibody (AQP4) were measured by Elisa. Results: the ratio of cTfh and B cells and the level of IL-21 increased significantly in patients with remission and healthy persons, but there was no significant difference between the patients in remission and healthy people in the ratio of cTfh / cTfr and the level of IL-21. There was no significant difference in AQP4-Ab between relapsed and remission patients. There was a positive correlation between cTfh ratio and B cell ratio, plasma IL-21 level, and a negative correlation between cTfr ratio and IL-21 level. The ratio of cTfh/cTfr was positively correlated with the ratio of B cells and IL-21, but the level of AQP4-Ab was not correlated with the ratio of B cell to cTfh and the ratio of cTfh / cTfr and IL-21. Conclusion the changes of cTfh / cTfr ratio and the imbalance of cTfh/cTfr ratio in patients with cTfh / NMOSD may promote the abnormal activation of humoral immune response and thus participate in the pathogenesis of NMOSD.
【作者单位】：第四军医大学唐都医院神经内科;第四军医大学唐都医院心脏内科;第四军医大学校务部门诊部;
【基金】：国家自然科学基金资助项目(编号:81301022) 陕西省自然科学基础研究计划项目(编号:2016JM8033)
【分类号】：R744.52

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