星形胶质细胞的缝隙连接蛋白43参与冷冻伤后脑水肿的形成

发布时间：2018-05-12 11:38

本文选题：缝隙连接蛋白 + 星形胶质细胞　；参考：《中国病理生理杂志》2016年11期

【摘要】：目的:探讨脑水肿后星形胶质细胞缝隙连接蛋白43(Cx43)的表达及其在脑水肿的发生发展过程中所起的作用。方法:采用颅骨外液氮冷冻法建立大鼠右侧顶叶皮层脑水肿模型。实验分为假手术组、脑水肿模型组和脑水肿模型+缝隙连接阻断剂(carbenoxolone或octanol)干预组。干湿重法测定冷冻伤后的脑含水量;甲酰胺法测定大鼠血脑屏障通透性的改变;HE染色观察冷冻伤脑组织的病理变化;Western blot法和免疫组化检测Cx43蛋白的表达情况。结果:冷冻伤可引起大鼠损伤脑皮层区的含水量增加,在冷冻伤后24 h脑水肿发展到高峰。冷冻伤引起损伤脑皮层区域的血脑屏障通透性增加,范围大于直接冷冻损伤区。HE染色观察显示冷冻伤中心区细胞坏死明显,而冷冻伤周围区域出现水肿。脑冷冻伤引起冷冻伤周围皮层区域的Cx43蛋白表达增加,但冷冻伤中心区的Cx43蛋白表达降低。Carbenoxolone或octanol阻断Cx43的功能,降低了冷冻伤皮层区的含水量和血屏障通透性。结论:脑水肿时星形胶质细胞上的Cx43表达上调,功能增强;阻断Cx43的功能可在一定程度上减轻脑水肿。
[Abstract]:Aim: to investigate the expression of gap junction protein 43 (Cx43) in astrocytes after cerebral edema and its role in the development of brain edema. Methods: the right parietal cortex brain edema model was established by cryopreservation with extracranial liquid nitrogen. The experiment was divided into sham-operation group, brain edema model group and gap junction blocker carbenoxolone or octanolol intervention group. The changes of blood brain barrier permeability were measured by formamide method. The pathological changes of brain tissue were observed by HE staining. The expression of Cx43 protein was detected by Western blot and immunohistochemistry. Results: the water content in the cortical area of the injured rats was increased after freezing injury, and reached the peak at 24 hours after the injury. The permeability of blood-brain barrier in the injured cerebral cortex was increased by freezing injury, and the range was larger than that of the direct freezing injury. The results of HE staining showed that the cell necrosis was obvious in the central area of the injury, but edema appeared in the surrounding area of the injury. The expression of Cx43 protein increased in the cortical area around the frozen injury, but the expression of Cx43 protein in the central area of the frozen injury decreased. Carbenoxolone or octanol blocked the function of Cx43, and decreased the water content and blood barrier permeability of the cortical area. Conclusion: the expression of Cx43 in astrocytes is up-regulated and the function is enhanced during cerebral edema, and blocking the function of Cx43 can alleviate the brain edema to some extent.
【作者单位】：浙江中医药大学基础医学院;浙江大学城市学院医学院;浙江大学医学院基础医学系;
【基金】：浙江省教育厅科研项目(No.Y201120080) 浙江中医药大学校级科研基金(No.2014ZY02)
【分类号】：R742

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