M4型瞬时受体电位通道在蛛网膜下腔出血大鼠脑血流自主调节障碍中的作用

发布时间：2018-05-14 19:06

本文选题：蛛网膜下腔出血 + 瞬时受体电位通道　；参考：《中国脑血管病杂志》2017年05期

【摘要】：目的探讨M4型瞬时受体电位通道(TRPM4)在蛛网膜下腔出血(SAH)大鼠模型中对脑血流量自主调节障碍的作用。方法选择清洁级健康雄性SD大鼠120只,按随机数字表法分为假手术、SAH、阴性对照及治疗组,剔除死亡大鼠。采用立体定向仪鞍上池注射法建立SAH模型,分别向假手术组和阴性对照组注射等渗盐水0.2 ml,分别向SAH组和治疗组注射自体尾动脉血0.2 ml。通过置入式微量泵分别向假手术组和SAH组大鼠的侧脑室持续泵入等渗盐水,向阴性对照组和治疗组持续泵入浓度为0.03 mol/L的TRPM4阻滞剂(9-Phenanthrol),4组大鼠分别于第3、5和7天接受大脑皮质局部血流量和全脑血流量的检测。结果 120只SD大鼠中共有106只(88.3%)存活至研究时间点,4组分别以21只大鼠(各时点分别为7只)进行数据分析。第3、5、7天,假手术、SAH、阴性对照和治疗组大脑皮质局部和全脑血流量的差异均有统计学意义(均P0.05);SAH组皮质局部血流量[第3、5、7天分别为(141±18)、(148±24)、(168±19)PU]和全脑血流量[第3、5、7天分别为(93±5)、(85±5)、(85±6)ml/(100 g·min)]均较假手术组[皮质局部:(235±17)、(220±24)、(224±20)PU,全脑:(141±10)、(147±8)、(143±8)ml/100 g·min]明显降低(均P0.05),治疗组大脑皮质局部和全脑血流量[皮质局部:(183±26)、(173±26)和(187±15)PU,全脑:(114±10)、(104±9)和(119±5)ml/(100 g·min)]均较SAH组明显增加(均P0.05)。结论 TRPM4对改善SAH后脑血流自主调节障碍有明显作用。
[Abstract]:Objective to investigate the effect of M4 transient receptor potential channel TRPM4 on cerebral blood flow autonomic regulation in SAH rat model of subarachnoid hemorrhage. Methods 120 healthy male Sprague-Dawley rats of clean grade were randomly divided into three groups: sham-operated group, negative control group and treatment group. The SAH model was established by injecting the suprasellar cistern with stereotactic instrument. The sham-operated group and the negative control group were injected with 0.2 ml of isosmotic saline, and 0.2 ml of autologous caudate artery blood were injected into the SAH group and the treatment group respectively. Isoosmotic saline was continuously pumped into the lateral ventricle of rats in sham-operation group and SAH group by implanting micropump. The regional cerebral cortex blood flow and the whole brain blood flow were measured on the 5th and 7th day after continuous infusion of 0.03 mol/L TRPM4 blocker into the negative control group and the treatment group. Results A total of 106 SD rats (88. 3%) survived to the time point of the study. The data of 21 rats (7 rats at each time point) were analyzed. And on the third day of the fifth day, There were statistically significant differences in regional and global cerebral blood flow between the sham operation group and the treatment group (P 0.05). The regional cerebral blood flow was significantly higher in the SAH group [141 卤18 19)PU on the 3rd day, 148 卤24 19)PU] and 93 卤5 卤5 6)ml/(100 g on the 357 day respectively) in the sham-operated group and in the treatment group (P < 0.05), which were significantly higher than those in the control group (P < 0.05). The total cerebral blood flow was significantly higher in the control group than that in the control group (P < 0.05), and the total cerebral blood flow volume in the treatment group was significantly higher than that in the control group (P < 0.05) and the total cerebral blood flow volume in the treatment group (P < 0.05). In the sham-operated group, the local cerebral blood flow in the cortical cortex was significantly lower than that in the SAH group (P 0.05). The local cerebral blood flow in the treatment group was significantly higher than that in the SAH group (183 卤26173 卤26173 卤26) and 187 卤15 5)ml/(100 / min, and the whole brain was 114 卤104.104 卤9 and 119 卤5)ml/(100 / g / min, respectively, in the sham operation group (all P0.05 卤0. 05 卤0. 05 5)ml/(100 / min). The blood flow was significantly higher in the sham-operated group than in the control group (P < 0.05), but it was significantly higher in the sham-operation group than in the control group (P < 0.05), and was significantly higher in the sham-operated group than in the control group (P < 0.05), and was significantly higher than that in the control group (P < 0.05). Conclusion TRPM4 plays an important role in improving cerebral blood flow autonomic regulation after SAH.
【作者单位】：昆明医科大学第一附属医院神经外二科;
【分类号】：R743.35

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