STAT3、β-catenin和VEGF在胶质瘤中的表达、相关性及临床意义

发布时间：2018-05-15 12:51

本文选题：STAT3 + β-catenin　；参考：《苏州大学》2014年硕士论文

【摘要】：目的研究STAT3、β-catenin和VEGF在胶质瘤组织及正常脑组织中的蛋白表达，探讨三者在人脑胶质细胞瘤发生、发展、增殖和浸润中的作用，分析三者与胶质瘤临床观察指标（性别、年龄、肿瘤发生部位和胶质瘤WHO分级）的关系以及三者在胶质瘤组织中表达的相关性，从而指导胶质瘤恶性程度的判断和预后评估，为寻找胶质瘤靶向治疗的新靶点提供理论基础。方法收集徐州医学院附属连云港第一人民医院病理科2009年1月～2012年12月胶质瘤存档腊块标本106例，均采集于存档病例。其中，男性62例，女性44例，男女性别比为1.4:1；年龄最小者为11岁，最大者为78岁，平均年龄46.4岁。106例脑胶质瘤患者病理资料：所有胶质瘤标本均经神经外科手术切除所得且已经病理诊断证实，并由连云港第一人民院病理科诊断医师按中枢神经系统肿瘤WHO分类最新标准（2007年）分为Ⅰ、Ⅱ、Ⅲ、Ⅳ级。低级别组(Ⅰ、Ⅱ级)41例，高级别组(Ⅲ、Ⅳ级)65例；其中Ⅰ级为13例，Ⅱ级为28例，Ⅲ级为31例，Ⅳ级34例。对照组10例正常脑组织标本通过颅内减压手术获得。实验采用免疫组织化学Envision法检测STAT3、β-catenin及VEGF在人脑胶质瘤组织中的表达情况，结合临床病理资料进行统计分析，数据结果应用SPSS19.0统计软件处理，根据数据类型选用秩和检验及Spearman相关分析，以P＜0.05为差异有统计学意义。结果 1.STAT3在正常脑组织中未发现阳性表达，在胶质瘤组织中的阳性表达率为50.0%(53/106)，差异有统计学意义(P＜0.01)。STAT3表达与胶质瘤WHO分级相关，在Ⅰ、Ⅱ、Ⅲ、Ⅳ级中的阳性表达率分别为30.77%(4/13)，32.14%(9/28)，61.29%(19/31)，61.76%(21/34)，差异有统计学意义(P＜0.05)。STAT3表达与患者性别、年龄及肿瘤发生部位无相关性(P＞0.05)。 2.β-catenin在正常脑组织中未发现阳性表达，在胶质瘤组织中的阳性表达率为86.79%(92/106)，差异有统计学意义(P＜0.01)。β-catenin表达与胶质瘤WHO分级相关，在Ⅰ、Ⅱ、Ⅲ、Ⅳ级中的阳性表达率分别为61.54%(8/13)，82.14%(23/28)，90.32%(28/31)，97.06%(33/34)，差异有统计学意义(P＜0.01)。β-catenin表达与患者性别、年龄及肿瘤发生部位无相关性(P＞0.05)。 3.VEGF在正常脑组织中未发现阳性表达，在胶质瘤组织中的阳性表达率为58.49%(62/106)，差异有统计学意义(P＜0.01)。VEGF表达与胶质瘤WHO分级相关，在Ⅰ、Ⅱ、Ⅲ、Ⅳ级中的阳性表达率分别为15.38%(2/13)，39.29%(11/28)，58.06%(18/31)，91.18%(31/34)，差异有统计学意义(P＜0.01)。VEGF表达与患者性别、年龄及肿瘤发生部位无相关性(P＞0.05)。 4.在胶质瘤组织中，STAT3和β-catenin同为阳性表达53例，阴性表达14例，，二者呈正相关（r=0.801，P＜0.01）。STAT3和VEGF同为阳性表达50例，阴性表达41例，二者呈正相关（r=0.844，P＜0.01）。β-catenin和VEGF同为阳性表达62例，阴性表达14例，二者呈正相关（r=0.864，P＜0.01）。结论 1.STAT3在胶质瘤组织中的阳性表达与正常脑组织中的表达差异有统计学意义。STAT3在胶质瘤组织中的表达与WHO分级相关；而与研究对象的性别、年龄和肿瘤发生的部位三个临床观察指标之间的差异无统计学意义。 2.β-catenin在胶质瘤组织中的阳性表达与正常脑组织中的表达差异有统计学意义。β-catenin在胶质瘤组织中的表达与WHO分级相关；而与研究对象的性别、年龄和肿瘤发生的部位三个临床观察指标之间的差异无统计学意义。 3.VEGF在胶质瘤组织中的阳性表达与正常脑组织中的表达差异有统计学意义。VEGF在胶质瘤组织中的表达与WHO分级相关；而与研究对象的性别、年龄和肿瘤发生的部位三个临床观察指标之间的差异无统计学意义。 4.在胶质瘤组织中STAT3、β-catenin和VEGF的表达两两之间呈正相关。
[Abstract]:Objective to study the protein expression of STAT3, beta -catenin and VEGF in glioma and normal brain tissue, to explore the role of the three in the occurrence, development, proliferation and infiltration of human glioma, and to analyze the relationship between the three and the clinical observation of glioma (sex, age, tumor site and glioma WHO classification) and the three in glioma. The correlation expressed in tissues can guide the judgement of malignant degree and prognosis of gliomas, and provide a theoretical basis for finding new targets of glioma targeted therapy.
Methods 106 specimens of glioma deposited wax from January 2009 to December 2012, the first people's Hospital of Lianyungang, Xuzhou Medical College, were collected and collected in archived cases. Among them, 62 men and 44 women were male and female, the sex ratio of men and women was 1.4:1; the youngest was 11 years old, the largest was 78 years old, and the average age of 46.4 years of brain glioma patients was 46.4 years old. Pathological data: all glioma specimens were diagnosed by surgical excision in the Department of neurosurgery and confirmed by pathological diagnosis. According to the latest standard of WHO classification of central nervous system tumor WHO (2007), the diagnostics of the first people's Hospital of the first people's hospital were divided into 1, II, III, IV grade. 41 cases of low grade group (grade I, grade II), and 65 cases of advanced group (III, IV); Grade I was 13, class II was 28, grade III was 31, grade IV was 34. 10 normal brain tissue specimens from the control group were obtained through intracranial decompression. The immunohistochemical Envision method was used to detect the expression of STAT3, beta -catenin and VEGF in human glioma tissue, and the data were analyzed with clinicopathological data. The results should be obtained. The data were processed by SPSS19.0 statistical software. Rank sum test and Spearman correlation analysis were used according to data type. The difference between P < 0.05 was statistically significant.
Result
The positive expression of 1.STAT3 was not found in normal brain tissue. The positive expression rate in glioma tissues was 50% (53/106), and the difference was statistically significant (P < 0.01).STAT3 expression was related to the WHO classification of glioma. The positive expression rate in grade I, II, III, and IV was 30.77% (4/13), 32.14% (9/28), 61.29% (19/31), 61.76% (21/34), and the difference was unified. There was no correlation between the expression of.STAT3 (P < 0.05) and the sex, age and location of tumor (P > 0.05).
The positive expression rate of 2. beta -catenin in normal brain tissue was 86.79% (92/106), and the difference was statistically significant (P < 0.01). The expression of beta -catenin was associated with the WHO grading of glioma. The positive expression rates in grade I, II, III, and IV were 61.54% (8/13), 82.14% (23/28), 90.32% (28/31), 97.06% (33/34), respectively. The difference was statistically significant (P < 0.01). There was no correlation between the expression of beta -catenin and the sex, age and location of tumor (P > 0.05).
The positive expression of 3.VEGF was not found in normal brain tissue. The positive expression rate in glioma tissues was 58.49% (62/106), and the difference was statistically significant (P < 0.01).VEGF expression was related to the WHO classification of glioma. The positive expression rate in grade I, II, III, and IV was 15.38% (2/13), 39.29% (11/28), 58.06% (18/31), 91.18% (31/34), and the difference was unified. There was no correlation between the expression of.VEGF (P < 0.01) and the sex, age and location of tumor (P > 0.05).
4. in glioma tissue, STAT3 and beta -catenin were positive expression in 53 cases, negative expression in 14 cases, two positive correlation (r=0.801, P < 0.01).STAT3 and VEGF positive expression, 41 cases, two positive correlation (r=0.844, P < 0.01). 62 cases were positive expression of beta -catenin and VEGF, negative expression 14 cases, two positive correlation (r=0.864, P) < 0.01).
conclusion
The difference between the positive expression of 1.STAT3 in glioma tissues and the expression of normal brain tissue has statistical significance. The expression of.STAT3 in the glioma tissue is related to the WHO classification, but there is no significant difference between the three clinical observation indexes of the sex, age and the site of the tumor.
The positive expression of 2. beta -catenin in glioma tissues was significantly different from that in normal brain tissue. The expression of beta -catenin in glioma tissues was related to the WHO classification, but there was no significant difference between the sex, age and the site of the tumor. The difference was not statistically significant between the sex, age and the site of the tumor.
The difference between the positive expression of 3.VEGF in glioma tissues and the expression of normal brain tissue has statistical significance. The expression of.VEGF in the glioma tissue is related to the WHO classification, but there is no significant difference between the three clinical observation indexes of the sex, age and the site of the tumor.
4. there was a positive correlation between the expression of STAT3, beta -catenin and VEGF in glioma tissues 22.

【学位授予单位】：苏州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R739.41

【参考文献】