S-腺苷蛋氨酸通过降低Cav-1的表达保护KCl刺激引起的神经元损伤

发布时间：2018-05-16 11:36

本文选题：S-腺苷蛋氨酸 + 氯化钾　；参考：《中国药理学通报》2017年12期

【摘要】：目的探讨S-腺苷蛋氨酸(S-adenosylmethionine,SAM)对KCl刺激引起的原代培养的小鼠皮层神经元损伤是否有保护作用及机制。方法将原代培养1周的小鼠皮层神经元随机分为3组:正常(Control)组、KCl组、SAM-KCl组。用倒置显微镜观察各组细胞形态的变化,用q PCR、Western blot分别检测各组质膜微囊结构蛋白~(-1)(caveolin~(-1),Cav~(-1))mRNA及蛋白的表达变化。结果 0.05 mol·L~(-1)KCl作用于原代培养1周的小鼠皮层神经元12 h可使神经元产生明显的损伤,突起缩短变细,胞体变小,细胞聚团。0.02 mol·L~(-1)SAM预处理6 h可明显降低KCl引起的细胞损伤,q PCR、Western blot分析显示KCl可使原代培养1周的皮层神经元Cav~(-1) mRNA及蛋白表达增高(P0.01),SAM可降低KCl引起的神经元Cav~(-1) mRNA及蛋白表达增高(P0.05)。结论 SAM对KCl引起的神经元损伤有保护作用,该保护作用与其降低Cav~(-1)的表达有关。
[Abstract]:Objective to investigate the protective effect and mechanism of S-adenosylmethionine (S-adenosylmethionine) on primary cultured cortical neuron injury induced by KCl in mice. Methods the primary cultured cortical neurons were randomly divided into three groups: normal control group (KCl) group and SAM-KCl group. The morphologic changes of the cells in each group were observed by inverted microscope. The mRNA and protein expression of the plasma membrane microencapsulation protein (PMC) were detected by qPCRX Western blot in each group. Results the effects of 0. 05 mol L~(-1)KCl on primary cultured mouse cortical neurons for 12 h resulted in obvious damage to the neurons, the process was shortened and the body of the neurons became smaller. Cell cluster. 0.02 mol L~(-1)SAM pretreatment for 6 h significantly reduced cell damage induced by KCl. Western blot analysis showed that KCl could increase the expression of mRNA and protein in primary cultured cortical neurons for 1 week. Conclusion SAM has a protective effect on the neuronal injury induced by KCl, which is related to the decrease of Cavo 1 expression.
【作者单位】：徐州医科大学江苏省麻醉学重点实验室江苏省麻醉与镇痛应用技术重点实验室;
【基金】：国家自然科学基金资助项目(No 81500950)
【分类号】：R741

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