前扣带回内肿瘤坏死因子α过表达在神经病理性疼痛中的作用

发布时间：2018-05-18 14:38

本文选题：肿瘤坏死因子-α + 前扣带回　；参考：《中山大学学报(医学科学版)》2017年01期

【摘要】：【目的】探讨坐骨神经部分损伤对前扣带回(ACC)内致炎细胞因子TNF-α、IL-1β和抗炎细胞因子IL-10表达的影响,并分析它们在神经病理性疼痛中的可能作用。【方法】采用行为学、Western blot、免疫荧光组织化学方法,观察外周神经损伤后ACC内上述细胞因子表达的改变以及ACC内微量注射相应中和抗体对大鼠痛行为的影响。【结果】(1)坐骨神经部分损伤(SNI)诱导大鼠损伤侧触诱发痛并伴有ACC内致炎细胞因子TNF-α和抗炎细胞因子IL-10蛋白水平显著升高,其中TNF-α升高幅度更大,但对IL-1β的表达无影响;(2)免疫荧光双染结果表明SNI后ACC内上调的TNF-α以及IL-10都表达于神经元而非胶质细胞内;(3)通过SNI术前及术后连续多次在ACC内微量注射TNF-α中和抗体,显著缓解大鼠触诱发痛,但给予IL-1β中和抗体对大鼠痛行为无影响。【结论】以上结果表明坐骨神经损伤可能通过诱导ACC神经元内TNF-α异常分泌易化脊髓背角痛信息传入。
[Abstract]:[objective] to investigate the effects of partial sciatic nerve injury on the expression of inflammatory cytokines TNF- 伪 IL-1 尾 and anti-inflammatory cytokines (IL-10) in anterior cingulate gyrus (ACCs). The possible role of them in neuropathic pain was analyzed. [methods] Western blot and immunofluorescence histochemistry were used. To observe the changes of cytokines expression in ACC after peripheral nerve injury and the effect of microinjection of neutralizing antibody into ACC on the pain behavior of rats. [results] 1) sciatic nerve injury induced by sciatic nerve injury in rats. The levels of inflammatory cytokine TNF- 伪 and anti-inflammatory cytokine IL-10 protein in ACC were significantly increased. The increase of TNF- 伪 is greater than that of TNF- 伪. The results of immunofluorescence double staining showed that TNF- 伪 and IL-10 in ACC were up-regulated in neurons but not in glial cells after SNI. TNF- 伪 neutralizing antibody was microinjected into ACC repeatedly before and after SNI. The results indicated that sciatic nerve injury might facilitate the afferent of spinal dorsal horn pain information by inducing abnormal secretion of TNF- 伪 in ACC neurons.
【作者单位】：中山大学疼痛研究中心//中山医学院生理教研室;
【基金】：国家自然科学基金(81371228) 广东省科技计划项目(2012B031800254) 广东省自然科学基金(S2013010016647) 中央高校基本科研业务费专项资金(16ykjc02)
【分类号】：R741

【相似文献】