偏头痛患者血清S100B蛋白、NSE水平及其临床意义

发布时间：2018-05-18 20:29

本文选题：偏头痛 + S100B蛋白　；参考：《河北医科大学》2017年硕士论文

【摘要】：目的:偏头痛是神经内科的一种常见疾病,发病机制尚不十分清楚。最近的研究提出偏头痛在反复发作的基础上可能导致神经细胞的损伤,而并不是人们以往认为的一种良性功能性头痛疾病。Sangtec-I00B(S-100B)蛋白和神经元特异性烯醇化酶(NSE)作为中枢神经系统损伤的标志物,可反映神经胶质细胞和神经元的损伤,目前成为临床的研究热点之一并引起广大学者的关注。本研究旨在探讨偏头痛患者血清S100B蛋白、NSE水平及其临床意义,并分析了血清S100B蛋白、NSE水平与偏头痛病程、持续时间、发作频率、视觉模拟量表评分(VAS)及头痛影响测验(HIT-6)评分的相关性。以期进一步了解偏头痛的发病机制及是否存在脑损伤。方法:1选取2015年3月—2016年12月就诊于河北医科大学第二医院神经内科门诊的偏头痛患者53例,其中有先兆偏头痛患者20例,无先兆偏头痛患者33例。采用国际头痛疾病分类第三版(Beta版)中偏头痛诊断标准,选取河北医科大学第二医院体检中心的50例健康体检者为对照组。并对其一般资料、临床资料及流行病学资料进行采集。2在偏头痛发作间期采集所有入组病人的静脉血5 ml,离心后收集上层血清,低温保存至检测。S100B蛋白、NSE均采用酶联免疫吸附试验检测。3应用SPSS20.0软件进行统计学分析,比较偏头痛组与健康对照组间的差异,有先兆偏头痛组与无先兆组的差异,男性偏头痛患者与女性偏头痛患者的差异,分别分析血清S100B蛋白和NSE与偏头痛病程、持续时间、发作频率、VAS评分及HIT-6评分的相关性。结果:1偏头痛组血清S100B蛋白明显高于健康对照组,差异有统计学意义,(P0.01);偏头痛组血清NSE水平与对照组差异无统计学意义,(P0.05)。2有先兆和无先兆偏头痛组患者血清S100B蛋白差异无统计学意义,CP0.05);有先兆偏头痛组患者血清NSE水平虽高于无先兆偏头痛组患者,但未达到统计学意义,(P0.05)。3在健康对照组中男性与女性血清S100B蛋白、NSE差异均无统计学意义,(P0.05)。在偏头痛组中男性与女性患者血清S100B蛋白、NSE差异均无统计学意义,(P0.05)。4偏头痛组患者血清S100B蛋白、NSE水平与偏头痛患者的病程、持续时间、发作频率、VAS评分及HIT-6评分均无显著相关性。结论:本研究结果表明在偏头痛发作间期存在神经胶质细胞的激活或损伤,并存在血脑屏障(BBB)的功能障碍;同样提示在偏头痛发作间期并没有明确的神经元损伤。
[Abstract]:Objective: migraine is a common disease in neurology. Recent research suggests that migraine attacks on a recurring basis may lead to nerve cell damage. It is not a benign functional headache disease known as Sangtec-I00BFS-100B) and neuron specific enolase (NSE) as a marker of central nervous system injury, which can reflect the injury of glial cells and neurons. At present, it has become one of the focus of clinical research and attracted the attention of the majority of scholars. The purpose of this study was to investigate the serum S100B protein NSE level and its clinical significance in migraine patients, and to analyze the relationship between serum S100B protein NSE level and migraine duration, duration and frequency of attack. The correlation between visual analogue scale (VAS) and headache impact test (HIT- 6). In order to further understand the pathogenesis of migraine and whether there is brain damage. Methods from March 2015 to December 2016, 53 patients with migraine were selected from the Department of Neurology, second Hospital of Hebei Medical University, including 20 patients with aura migraine and 33 patients without aura migraine. Using the diagnostic criteria of migraine in the third edition of the International Classification of headache Diseases, 50 healthy people in the Center of physical examination of the second Hospital of Hebei Medical University were selected as the control group. The general data, clinical data and epidemiological data were collected. 2 the venous blood was collected from all the patients during the period of migraine attack, and the upper serum was collected after centrifugation. The difference between migraine group and healthy control group was compared between migraine group and healthy control group, and there was difference between aura migraine group and no aura group by enzyme linked immunosorbent assay (Elisa). The differences between male migraine patients and female migraine patients were analyzed. The correlation of serum S100B protein and NSE with migraine duration, frequency of attack and HIT-6 score were analyzed. Results the serum S100B protein in migraine group was significantly higher than that in healthy control group. There was no significant difference in serum NSE level between migraine group and control group. There was no significant difference in serum S100B protein between patients with migraine and without migraine, and NSE with migraine with aura. Although the level of migraine group without aura was higher than that of the patients without aura, However, there was no significant difference in serum S100B protein NSE between male and female in the healthy control group. There was no significant difference in serum S100B protein levels between male and female patients in migraine group. There was no significant correlation between the serum S100B protein level and the course, duration, frequency of attack and HIT-6 score of migraine patients. Conclusion: the results of this study indicate that there is activation or injury of glial cells and BBB dysfunction in migraine interictal period, and there is no definite neuronal injury during migraine interictal period.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R747.2

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