芹黄素抑制小胶质细胞活化作用研究

发布时间：2018-05-20 00:16

  本文选题：小胶质细胞 + 芹黄素　； 参考：《国际神经病学神经外科学杂志》2017年04期

【摘要】：目的探讨芹黄素对小胶质细胞活化的抑制作用。方法原代培养SD大鼠小胶质细胞,实验随机分为空白对照组、脂多糖组(LPS组)、LPS+芹黄素(10μM)组、LPS+芹黄素(20μM)组、LPS+芹黄素(50μM)组。MTT法检测芹黄素对小胶质细胞活性的影响;ELISA法检测IL-1、IL-10等炎症相关因子以及BDNF、PDNF等神经营养因子的表达;RTPCR、western blot检测炎症相关基因i NOS转录以及表达水平。结果 MTT检测结果表明,芹黄素对小胶质细胞活性无明显抑制。LPS刺激后,芹黄素预处理组IL-1等炎症因子表达水平明显低于单独LPS组(P0.05);而芹黄素预处理组IL-10的表达则高于单独LPS组(P0.01);与此同时,BDNF、PDNF等神经营养因子的分泌也有相同趋势(P0.05)。芹黄素预处理组i NOS表达和转录水平也低于单独LPS组(P0.05)。结论芹黄素可抑制活化状态小胶质细胞炎症因子以及炎症相关蛋白的表达,并可同时促进与神经元分化成熟相关神经营养因子的分泌。
[Abstract]:Objective to investigate the inhibitory effect of celanthin on the activation of microglia. Methods Sprague-Dawley rat microglia cells were cultured in primary culture and were randomly divided into blank control group. Effects of lipopolysaccharide (LPS) on lipopolysaccharide (LPS) group (10 渭 M) lipopolysaccharide (LPS) celanthin (20 渭 M) group (lipopolysaccharide) group (50 渭 M). MTT assay was used to detect the effect of celanthin on microglial activity. Elisa was used to detect the expression of inflammatory related factors such as IL-1, IL-10 and neurotrophic factors such as BDNF PDNF by RT PCR western blot. I NOS transcription and expression level were measured. Results the results of MTT showed that celanthin did not inhibit the activity of microglia. The expression of IL-1 and other inflammatory factors were significantly lower in the celanthin pretreatment group than in the LPS group, while the expression of IL-10 in the carvingin preconditioning group was higher than that in the LPS group, and there was a similar trend in the secretion of neurotrophic factors such as P0.05 in the LPS preconditioning group. The expression and transcription level of I NOS in celanthin preconditioning group was also lower than that in LPS group alone (P 0.05). Conclusion celanthin can inhibit the expression of inflammatory cytokines and inflammatory related proteins in activated microglia and promote the secretion of neurotrophic factors related to neuronal differentiation and maturation at the same time.
【作者单位】： 昆明医科大学第一附属医院;云南省干细胞和再生医学重点实验室;
【基金】：国家自然科学基金(81241126,81360197) 云南省教育厅科学研究基金项目(2013C227)
【分类号】：R742.5
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