肢体缺血后处理对脑缺血大鼠神经功能恢复和神经细胞增殖的作用

发布时间：2018-05-20 23:10

本文选题：脑缺血 + 缺血后处理　；参考：《四川大学学报(医学版)》2017年03期

【摘要】：目的探索非侵入式肢体缺血后处理(NLIP)对脑缺血大鼠的脑保护作用和损伤侧皮质区神经细胞的表达与分布。方法 SD大鼠110只随机分成假手术组(n=10),缺血再灌注损伤组(I/R组,n=50),NLIP组(治疗组,n=50)。I/R组和治疗组动物采用线栓法制备大脑中动脉栓塞(MCAO)模型(假手术组不插入线栓),治疗组于再灌注即刻给予NLIP处理(改良的缺血弹性带股动脉近端加压阻断股动脉10 min,再通10 min,循环3次),术后3d、7d、14d、21d、28d分别称取大鼠体质量,并进行行为学评估后处死大鼠取脑组织,免疫组织化学染色检测各时点缺血脑皮质中的双皮质素(DCX)和胶质细胞原纤维酸性蛋白(GFAP)的表达,Western blot检测缺血脑皮质中微管相关蛋白2(MAP2)的表达。结果假手术组动物术后1~3d表现出轻度的体质量下降和行为学缺损,术后1d出现上肢肌力减弱,之后开始恢复。与假手术组比较,以上神经缺损在I/R组和治疗组动物中更加明显(P0.05),术后3d到21d逐渐缓解,到术后28d时接近假手术组水平。与I/R组相比,治疗组动物体质量在术后2~7d有明显恢复(P0.05),动物上肢肌力在14~28d有明显改善(P0.05),治疗组行为学评分在各时点与I/R组差异无统计学意义。与假手术组比较,I/R组和治疗组脑皮质缺血区周围DCX阳性细胞和GFAP阳性细胞随时间推移逐渐增多,伴随胞体肥大和突起变长加粗,分别在术后21d和28d大量增殖并聚集在脑皮质缺血区周围。I/R组和治疗组在术后3d缺血脑皮质中MAP2表达量下降,但与假手术组差异无统计学意义(P0.05),之后逐渐上调,术后14d和21d时高于假手术组(P0.05)。治疗组MAP2表达量在术后7~28d高于假手术组(P0.05),在术后7d时高于I/R组(P0.05)。结论 NLIP可减轻脑缺血大鼠神经行为学异常和促进缺血周围区神经细胞的增多。
[Abstract]:Objective to investigate the protective effect of NLIP on the brain and the expression and distribution of neurons in the injured lateral cortex in rats with cerebral ischemia. Methods 110 Sprague-Dawley rats were randomly divided into sham operation group (n = 10) and ischemia / reperfusion injury group (n = 50 / R group). The treatment group was treated with NLIP immediately after reperfusion (modified ischemic elastic band femoral artery proximal end compression was blocked for 10 min, re-opened for 10 min, circulatory 3 times, 3 days after operation, 7 days, 14 days, 21 days, 28 days, respectively, the weight of the rats was taken. After behavioral evaluation, the rats were killed to take brain tissue, Immunohistochemical staining was used to detect the expression of DCX) and glial fibrillary acidic protein (GFAP) in ischemic cerebral cortex. Western blot was used to detect the expression of microtubule-associated protein 2map2 (MAP2) in ischemic cerebral cortex. Results in the sham-operated group, the body mass decreased slightly and the behavioral defect appeared on the 3rd day after operation, and the muscle strength of the upper limb decreased on the 1st day after the operation, and then it began to recover. Compared with the sham operation group, the above nerve defects were more obvious in the I / R group and the treatment group than in the control group, and gradually relieved from 3 to 21 days after operation, and were close to the level of the sham operation group at 28 days after operation. Compared with the I / R group, the animal mass of the treatment group recovered significantly on the 2nd day, 7th day after operation, and the muscle strength of the upper limb improved significantly on the 14th and 28th days. There was no significant difference in the behavioral score between the treatment group and the I / R group at all time points. Compared with the sham-operated group, the DCX positive cells and GFAP positive cells in the ischemic area of the cerebral cortex increased gradually with the increase of the cell body hypertrophy and the thickening of the processes in the I / R group and the treatment group. On the 21st and 28th days after operation, the expression of MAP2 in the ischemic cortex decreased in the I- / R group and the treatment group on the 3rd day after operation, but there was no significant difference between the control group and the sham-operated group (P 0.05), and then increased gradually. At 14 and 21 days after operation, it was higher than that in sham operation group (P 0.05). The expression of MAP2 in the treatment group was higher than that in the sham-operated group on the 7th day after operation (P 0.05), and at the 7th day after operation, it was higher than that in the I / R group (P 0.05). Conclusion NLIP can attenuate the neurobehavioral abnormality and promote the increase of nerve cells in the ischemic area in rats with cerebral ischemia.
【作者单位】：四川大学华西医院康复医学科;四川省康复医学重点实验室;四川大学华西医院公共实验技术中心;
【基金】：“十二·五”国家科技支撑计划项目(No.2011BAI08B11) 青年科学基金(No.81401858)资助
【分类号】：R743.3

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