耳穴区经皮电刺激对颞叶癫痫大鼠癫痫发作频率及海马区白介素-1β、肿瘤坏死因子-α表达的影响

发布时间：2018-05-23 14:15

本文选题：颞叶癫痫 + 经皮耳穴电刺激　；参考：《针刺研究》2016年04期

【摘要】：目的:通过观察经皮电刺激耳迷走神经支配的耳穴区对颞叶癫痫大鼠自发癫痫发作频率及海马区炎性因子白细胞介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)蛋白及mRNA表达的影响,探讨耳穴刺激对颞叶癫痫的缓解效应及其可能的分子机制。方法:SD大鼠随机分为正常组、模型组、治疗组,每组12只。采用氯化锂-匹罗卡品腹腔注射复制慢性自发性颞叶癫痫大鼠模型,对治疗组大鼠耳迷走神经支配的"心""肺""皮质下"耳穴区进行经皮电刺激干预,每日1次,每次20min,连续6周。用长时程视频监测动物的癫痫发作情况,治疗后采用免疫荧光与荧光定量real-time PCR方法观察癫痫大鼠海马区IL-1β、TNF-α蛋白及mRNA表达情况。结果:颞叶癫痫大鼠有反复自发的癫痫发作,模型组大鼠的自发癫痫发作频率随时间延长有增加的趋势,治疗组大鼠的自发癫痫发作频率治疗后较治疗前显著减少(P0.05)。与正常组比较,模型组大鼠海马CA 1、CA 3区IL-1β、TNF-α免疫活性增强,免疫阳性细胞数明显增多(P0.05),海马内IL-1β、TNF-αmRNA表达水平显著上调(P0.01);与模型组比较,治疗组大鼠海马CA 1、CA 3区IL-1β与TNF-α蛋白的免疫活性降低,免疫阳性细胞数明显减少(P0.05),海马内IL-1β、TNF-αmRNA表达水平显著下调(P0.05,P0.01)。结论:耳迷走神经支配的耳穴区经皮刺激干预能显著降低颞叶癫痫大鼠反复自发的癫痫频率,此效应可能与下调海马区炎性因子IL-1β、TNF-α蛋白的免疫反应及mRNA表达水平有关,即经皮耳迷走神经刺激的抗癫痫效应可能与其海马内的抗炎作用有关。
[Abstract]:Objective: to observe the effects of transcutaneous stimulation of auricular points innervated by vagus nerve on the frequency of spontaneous seizure and the expression of IL-1 尾, tumor necrosis factor- 伪 (TNF- 伪) protein and mRNA in hippocampal area of rats with temporal lobe epilepsy by interleukin-1 尾 -interleukin-1 尾 in hippocampal region. To investigate the effect of auricular point stimulation on temporal lobe epilepsy and its possible molecular mechanism. Methods: Sprague-Dawley rats were randomly divided into normal group, model group and treatment group with 12 rats in each group. The rat model of chronic spontaneous temporal lobe epilepsy was induced by intraperitoneal injection of lithium-pilocarpine. The auricular region of the auricular vagus nerve innervated in the treatment group was treated with percutaneous electrical stimulation once a day. Each time 20 mins, 6 weeks in succession. After treatment, the expression of IL-1 尾 -TNF- 伪 protein and mRNA in hippocampal area of epileptic rats were observed by immunofluorescence and fluorescence quantitative real-time PCR. Results: there were recurrent spontaneous seizures in temporal lobe epilepsy rats. The frequency of spontaneous epileptic seizures in the model group increased with the prolongation of time. The frequency of spontaneous epileptic seizures in the treatment group was significantly lower than that in the pre-treatment group (P 0.05). Compared with the normal group, the immunoreactivity of IL-1 尾 -TNF- 伪 in the CA _ 1 / CA _ 3 region of the model group was increased, the number of immunoreactive cells was significantly increased, and the expression of IL-1 尾 -TNF- 伪 mRNA in the hippocampus was significantly up-regulated in the model group, compared with the model group, and compared with the model group, the expression of IL-1 尾 -TNF- 伪 was significantly increased in the model group. In the treatment group, the immunoreactivity of IL-1 尾 and TNF- 伪 protein in CA1 / CA3 region decreased, the number of immunopositive cells decreased significantly, and the expression of IL-1 尾 -TNF- 伪 mRNA in hippocampus decreased significantly. Conclusion: percutaneous stimulation of auricular acupoints innervated by auricular vagus nerve can significantly reduce the frequency of recurrent spontaneous epilepsy in temporal lobe epilepsy rats. This effect may be related to the down-regulation of immunoreaction and mRNA expression of IL-1 尾 TNF- 伪 protein in hippocampal area. The antiepileptic effect of transcutaneous vagus stimulation may be related to its anti-inflammatory effect in hippocampus.
【作者单位】：山东中医药大学针灸推拿学院;中国中医科学院针灸研究所生物化学与分子生物学研究室;长春中医药大学;山东省精神卫生中心;
【基金】：国家自然科学基金面上项目(No.81273833);国家自然科学基金青年科学基金项目(No.81303055)
【分类号】：R742.1

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