MiR-26b通过下调COX-2表达抑制神经胶质瘤的增殖、侵袭和迁移

发布时间：2018-05-24 01:13

本文选题：miR-b + 环氧化酶-　；参考：《中南大学学报(医学版)》2017年02期

【摘要】：目的:观察不同级别神经胶质瘤中miR-26b和环氧化酶(COX)-2的表达情况,研究miR-26b对于神经胶质瘤细胞增殖、侵袭和迁移的影响。方法:运用Western印迹和实时定量荧光PCR(q RT-PCR)检测不同级别神经胶质瘤中miR-26b和COX-2的表达情况;使用miR-26b mimic转染人神经胶质瘤细胞U87,上调miR-26b的表达;q RT-PCR检测miR-26b mimic转染后miR-26b和COX-2 mRNA的表达变化情况;双荧光素酶报告基因系统检测miR-26b对COX-2转录活性的影响;使用Transwell侵袭实验检测miR-26b对人神经胶质瘤细胞U87侵袭能力的影响;使用划痕实验检测miR-26b对人神经胶质瘤细胞U87迁移能力的影响;使用CCK-8(cell counting kit-8)检测miR-26对人神经胶质瘤细胞U87增殖能力的影响;裸鼠体内成瘤实验检测过表达miR-26b后胶质瘤细胞成瘤能力的变化。结果:随着神经胶质瘤肿瘤级别的增加,miR-26b表达明显降低(P0.05),而COX-2明显增加,差异有统计学意义(P0.001);双荧光素酶实验证实miR-26b可以直接靶向调控COX-2的蛋白表达水平;使用miR-26b mimic明显上调miR-26b的表达(P0.05);上调miR-26b可以显著降低COX-2的表达(P0.05);上调miR-26b可以抑制神经胶质瘤细胞增殖、侵袭和迁移能力(P0.05)。实验组和对照组相比肿瘤的质量和体积都变小。结论:随着神经胶质瘤肿瘤级别的增加,miR-26b表达逐渐降低,而COX-2表达逐渐增加。miR-26b可通过抑制COX-2表达从而抑制神经胶质瘤的增殖、侵袭和迁移。
[Abstract]:Aim: to observe the expression of miR-26b and cyclooxygenase COX-2 in gliomas and to investigate the effects of miR-26b on the proliferation, invasion and migration of glioma cells. Methods: the expression of miR-26b and COX-2 in gliomas of different grades were detected by Western blot and real-time fluorescence PCR(q RT-PCR. MiR-26b mimic transfected human glioma cell line U87 was used to up-regulate the expression of miR-26b and detect the changes of miR-26b and COX-2 mRNA expression after miR-26b mimic transfection, and the effect of miR-26b on COX-2 transcription activity was detected by double luciferase reporter gene system. Transwell invasion assay was used to detect the effect of miR-26b on the invasion ability of human glioma cell line U87, and scratch test was used to detect the effect of miR-26b on the migration ability of human glioma cell line U87. CCK-8(cell counting kit-8) was used to detect the effect of miR-26 on the proliferation of human glioma cell line U87, and the tumorigenic ability of human glioma cell line U87 was detected by tumorigenic assay in nude mice after the expression of miR-26b. Results: with the increase of tumor grade of glioma, the expression of miR-26b decreased significantly, while the expression of COX-2 increased significantly (P 0.001). Double luciferase assay confirmed that miR-26b could directly target the expression of COX-2 protein. The expression of miR-26b was significantly up-regulated by miR-26b mimic, the expression of COX-2 was significantly decreased by upregulation of miR-26b, and the proliferation, invasion and migration of glioma cells was inhibited by up-regulation of miR-26b. The mass and volume of the tumor in the experimental group were smaller than that in the control group. Conclusion: with the increase of glioma grade, the expression of miR-26b decreases gradually, while the expression of COX-2 increases gradually. MiR-26b inhibits the proliferation, invasion and migration of gliomas by inhibiting the expression of COX-2.
【作者单位】：海南医学院第一附属医院神经外科;中山大学孙逸仙纪念医院神经外科;
【基金】：海南省自然科学基金(20168298)~~
【分类号】：R739.41

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