内皮素-1在红藻氨酸致癫痫大鼠海马区的表达及依达拉奉的干预作用

发布时间：2018-05-28 15:14

本文选题：癫痫 + 内皮素(ET)-　；参考：《中国老年学杂志》2017年09期

【摘要】：目的观察内皮素(ET)-1在红藻氨酸(KA)致癫痫大鼠海马的表达及依达拉奉对其影响。方法 50只雄性Wistar大鼠被随机分成5组,每组10只。正常组、磷酸盐缓冲液(PBS)组、癫痫组,治疗组1(地西泮治疗),治疗组2(地西泮+依达拉奉治疗)。癫痫组在大鼠右侧海马以每公斤体重注射1.5μg/μl KA,PBS组采取同样的方法在相同部位注入等量PBS,治疗组1在癫痫模型成立后给予10 mg/kg地西泮终止癫痫,治疗组2在治疗组1的基础上1 h后再给予依达拉奉30 mg,1次/12 h,正常组不注射任何药物。结果激光共聚焦显微镜观察,癫痫组ET-1的表达密度显著高于PBS组及正常组(P0.05),治疗组较正常组、PBS组及癫痫组表达显著下降(P0.05),治疗组1较治疗组2表达显著增高(P0.05)。十二烷基硫酸钠、聚丙烯酰胺凝胶电泳(SDS-PAGE)分析显示ET-1的相对分子质量为2 492 D,其在癫痫组的表达较正常组及PBS组显著增加,治疗组较癫痫组显著降低(均P0.05),与激光共聚焦的结果相一致。结论 ET-1表达增加对癫痫后大鼠海马的神经元损伤具有细胞保护作用。
[Abstract]:Aim to observe the expression of endothelin (et)-1 in hippocampus of epileptic rats induced by kainic acid (kainic acid) and the effect of Edaravone on it. Methods 50 male Wistar rats were randomly divided into 5 groups with 10 rats in each group. Normal group, phosphate buffer solution PBS group, epilepsy group, treatment group 1 (diazepam treatment group, treatment group 2) (diazepam Edaravone treatment group). In the epileptic group, 1.5 渭 g / 渭 l Ka-PBS was injected into the right hippocampus of the rats at the right side of the body weight, and the same amount of PBSs was injected at the same site in the control group. In the treatment group 1, 10 mg/kg diazepam was given to terminate the epilepsy after the establishment of the epileptic model. Treatment group 2 was treated with Edaravone 30 mg / 12 h after 1 hour on the basis of treatment group 1, and no drug was injected into normal group. Results the expression density of ET-1 in epileptic group was significantly higher than that in PBS group and normal group (P0.05A). The expression of P0.05 in treatment group was significantly lower than that in control group and epilepsy group. The expression of ET-1 in treatment group 1 was significantly higher than that in treatment group 2 (P 0.05). Sodium dodecyl sulfate and polyacrylamide gel electrophoresis showed that the relative molecular weight of ET-1 was 2 492D, and its expression in epileptic group was significantly higher than that in normal group and PBS group. The treatment group was significantly lower than the epileptic group (P 0.05), which was consistent with the results of laser confocal focus. Conclusion the increased expression of ET-1 has a cytoprotective effect on hippocampal neuronal injury after epilepsy in rats.
【作者单位】：齐齐哈尔医学院第一附属医院神经内科;
【基金】：黑龙江省自然科学基金资助项目(No.H201498)
【分类号】：R742.1

【相似文献】