依达拉奉通过Notch信号通路调节BV-2小胶质细胞激活的研究

发布时间：2018-05-28 17:15

本文选题：脂多糖 + Notch信号通路　；参考：《神经解剖学杂志》2017年05期

【摘要】：目的:探讨Notch信号通路在激活的BV-2小胶质细胞的表达及依达拉奉对其表达的影响。方法:体外培养BV-2小胶质细胞系,将其分为对照组、脂多糖(lipopolysaccharide,LPS)激活处理3 h组和依达拉奉预处理1 h+LPS激活3 h组,采用免疫荧光双标染色及蛋白质印迹法检测Notch信号通路相关蛋白在激活的BV-2小胶质细胞的表达变化,包括Notch-1及下游的细胞内Notch受体结构域(intracellular Notch receptor domain,NICD)、重组结合蛋白抑制子(recombining binding protein suppressor of hairless,RBP-JK)、转移因子发卡和split-1增强子(transcription factor hairy and enhancer of split-1,Hes-1),以及依达拉奉干预后的影响。结果:免疫荧光双标结果显示,LPS激活的BV-2小胶质细胞Notch信号通路相关蛋白(Notch1、NICD、RBP-JK、Hes-1)荧光表达出现明显增强,依达拉奉可显著减少激活的BV-2小胶质细胞上述蛋白的荧光表达。Western Blot结果也显示依达拉奉可明显抑制激活的小胶质细胞Notch1、NICD、RBP-JK、Hes1的蛋白表达,结果具有统计学意义。结论:依达拉奉可通过Notch信号通路调节小胶质细胞的激活,从而影响其功能活动。
[Abstract]:Aim: to investigate the expression of Notch signaling pathway in activated BV-2 microglia and the effect of Edaravone on its expression. Methods: BV-2 microglia cell lines were cultured in vitro and divided into control group, lipopolysaccharide (LPS) activation treatment group for 3 h and Edaravone pretreatment 1 h LPS activation group for 3 h. The expression of Notch signaling pathway related proteins in activated BV-2 microglia was detected by double immunofluorescence staining and Western blotting. These include Notch-1 and its downstream intracellular Notch receptor domain, recombining binding protein suppressor of hairless RBP-JKN, transcription factor hairy and enhancer of split-1hes-1hes-1s, and the effect of Edaravone on it. Results: the results of double immunofluorescence showed that the fluorescence expression of LPS-activated BV-2 microglia Notch signal pathway related protein (NICDP-JKP- Hes-1) was significantly increased. Edaravone significantly reduced the fluorescence expression of the above mentioned protein in activated BV-2 microglia. Western Blot results also showed that Edaravone could significantly inhibit the expression of Notch1NICDP-JKHes1 protein in activated microglia cells, and the results were statistically significant. Conclusion: Edaravone can regulate the activation of microglia through Notch signaling pathway and affect its function.
【作者单位】：昆明医科大学基础医学院人体解剖学与组织胚胎学系;昆明医科大学科研实验中心;昆明医科大学海源学院;
【基金】：云南省应用基础研究计划重点项目(2015FA020) 国家自然科学基金(31260254) 云南省教育厅科学研究基金项目(2015C015Y)
【分类号】：R741

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