ERK信号通路抑制剂U0126对蛛网膜下腔出血大鼠早期脑损伤及神经元自噬的影响

发布时间：2018-05-29 09:52

  本文选题：蛛网膜下腔出血 + 早期脑损伤　； 参考：《西安交通大学学报(医学版)》2017年01期

【摘要】：目的探讨ERK信号通路特异性抑制剂U0126对蛛网膜下腔出血(SAH)对早期脑损伤及海马区神经细胞自噬的作用。方法成年雄性SD大鼠48只,随机数字表法分为对照组、SAH组、DMSO+SAH(二甲基亚砜)组、U0126+SAH组(ERK信号通路特异性抑制剂),共4组,每组各12只。采用血管内穿刺法(PIC)法制作SAH模型,分别于造模前30min经尾静脉注射等量的生理盐水、DMSO、U0126溶液0.5mL/只,于24h处死。干湿重法测量脑组织水含量,HE染色观察海马CA1区神经细胞形态结构变化;免疫组化及Western bloting检测海马区ERK及Beclin-1和LC3-Ⅱ表达水平的变化。结果与Sham组比较,SAH模型组脑组织含水量明显增加,大鼠海马CA1区神经元数量明显减少(P0.05),ERK及自噬相关因子Beclin-1和LC3-Ⅱ的表达明显高于对照组(P0.05);与SAH模型组比较,U0126组脑组织含水量明显增多,海马CA1区神经元数量明显较SAH组减少(P0.05),ERK信号信号通路被抑制,相应自噬相关因子Beclin-1和LC3-Ⅱ的表达降低(P0.05)。结论ERK信号通路抑制剂U0126可以抑制神经细胞自噬,加重SAH的早期脑损伤。
[Abstract]:Objective to investigate the effects of ERK signal pathway specific inhibitor U0126 on early brain injury and hippocampal neuronal autophagy in patients with subarachnoid hemorrhage. Methods Forty-eight adult male Sprague-Dawley rats were randomly divided into 4 groups (12 rats in each group) with DMSO SAH (dimethyl sulfoxide) group and U0126 SAH group. The SAH model was made by intravascular puncture method. 30min was injected into the tail vein with the same amount of 0.5mL/ solution DMSOSOU0126, and was killed at 24 hours. The changes of neuronal morphology and structure of hippocampal CA1 were observed by HE staining, and the changes of ERK, Beclin-1 and LC3- 鈪，

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