治疗干预对大鼠结核性脑膜炎蛛网膜颗粒结构的影响

发布时间：2018-06-06 20:03

本文选题：大鼠 + 结核性脑膜炎　；参考：《井冈山大学学报(自然科学版)》2016年06期

【摘要】：目的观察药物治疗干预措施对结核性脑膜炎大鼠蛛网膜颗粒结构的影响,为研究结核性脑膜炎继发脑积水形成机制和药物干预治疗提供理论依据。方法建立大鼠结核性脑膜炎模型,分为A、B、C、D组(实验组),实验A、B、C组均给予异烟肼及利福平抗结核治疗,其中B、C组分别另外经小脑延髓池注射尿激酶(B)、乌司他丁(C),D组为非治疗干预组,E组为健康空白对照组。第3周,处死大鼠,之前经小脑延髓池注入亚甲蓝定位蛛网膜颗粒,使用TUNEL法观察5组大鼠蛛网膜颗粒细胞凋亡情况差异。结果 A、B、C组蛛网膜颗粒中偶见细胞凋亡,非治疗干预D组蛛网膜颗粒中凋亡细胞增多,对照组未见凋亡。结论大鼠结核性膜炎后存在广泛的蛛网膜下腔炎症反应过程,使蛛网膜颗粒出现过度的细胞凋亡,治疗干预可能减少蛛网膜颗粒细胞凋亡。
[Abstract]:Objective to observe the effect of drug therapy intervention on arachnoid granule structure in rats with tuberculous meningitis and to provide theoretical basis for studying the formation mechanism of hydrocephalus secondary to tuberculous meningitis and drug intervention therapy. Methods the model of tuberculous meningitis was established in rats. The rats were divided into two groups: group D (experimental group) and group A (group C) were treated with isoniazid and rifampicin. In group C, urokinase BX was injected into cerebellar medullary cistern separately, and group D was treated with ulinastatin. Group E was healthy blank control group. At the third week, the rats were killed and methylene blue was injected into the cerebellar medulla oblongata cistern to locate the arachnoid granules. The apoptosis of arachnoid granulosa cells was observed by Tunel method. Results apoptosis was occasionally found in arachnoid granules in group A BX C and increased in group D without treatment, but no apoptosis was found in control group. Conclusion there is an extensive process of subarachnoid inflammatory reaction after tuberculous meningitis in rats, which leads to excessive apoptosis of arachnoid granules, and treatment intervention may reduce the apoptosis of arachnoid granulosa cells.
【作者单位】：江西省胸科医院神经内科;江西省第三人民医院神经内科;
【基金】：江西省自然科学基金项目(20114BAB205060)
【分类号】：R529.3

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