全细胞膜片钳技术检测化学损伤致痫大鼠海马脑片电生理特性

发布时间：2018-06-07 01:09

本文选题：全细胞膜片钳 + 癫痫　；参考：《中国老年学杂志》2016年21期

【摘要】：目的应用全细胞膜片钳技术检测化学损伤致痫大鼠海马脑片神经元基本电生理特性。方法将成年SD大鼠建立氯化锂-匹罗卡品模型后,急性分离获得海马脑片,应用全细胞膜片钳电流钳技术实时观察化学损伤致痫大鼠海马脑片CA1区锥体神经元膜电位及其单位时间内动作电位频率的变化情况;通过全细胞膜片钳电压钳技术,检测化学损伤后大鼠海马脑片CA1区锥体神经元的诱发兴奋性突触后电流的变化情况。结果急性分离所得海马脑片CA1区锥体神经元的胞体饱满并可见突起。可在大鼠海马脑片CA1区锥体神经元记录到自发的动作电位及刺激诱发兴奋性突触后电流,且化学损伤后神经元膜电位绝对值降低,动作电位频率加快,诱发的兴奋性突触后电流幅值升高。结论全细胞膜片钳技术可以用于癫痫大鼠急性分离所得海马脑片的电生理研究,化学损伤后神经元兴奋性明显升高,为开展癫痫相关的功能研究提供了新的途径。
[Abstract]:Objective to detect the basic electrophysiological characteristics of hippocampal neurons in epileptic rats induced by chemical injury by whole cell patch clamp technique. Methods the adult SD rat model of lithium-pilocarpine was established and the hippocampal slices were obtained. The changes of membrane potential and action potential frequency of pyramidal neurons in the CA1 region of hippocampal slices of epileptic rats induced by chemical injury were observed by whole-cell patch clamp current clamp technique. The changes of excitatory postsynaptic currents in CA1 pyramidal neurons in the hippocampal slices of rats were measured after chemical injury. Results the pyramidal neurons in the CA1 region of hippocampal slices were found to be plump and protruded. Spontaneous action potential and excitatory postsynaptic current were recorded in the pyramidal neurons of CA1 region in rat hippocampal slices. The absolute value of membrane potential decreased and the frequency of action potential increased after chemical injury. The amplitude of excitatory postsynaptic current was increased. Conclusion the whole-cell patch clamp technique can be used in the electrophysiological study of hippocampal slices isolated from epileptic rats, and the excitability of neurons is significantly increased after chemical injury, which provides a new approach for the study of epileptic-related function.
【作者单位】：遵义医学院附属医院神经内科;
【基金】：国家自然科学基金(81260201,81560224) 贵州省科学技术基金资助项目〔黔科合J字(2013)2327号〕遵义市优秀青年科技人才培养项目〔遵科合人(2014)10号〕贵州省高校优秀科技创新人才支持计划〔黔教合KY字(2014)247〕贵州省科技合作计划项目〔黔科合LH字(2015)7520号〕贵州省卫计委科技基金(gzwjkj 2015-1-052)
【分类号】：R742.1

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