川芎嗪对慢性压迫性损伤眶下神经致大鼠三叉神经痛的作用

发布时间：2018-06-07 23:54

本文选题：降钙素基因相关肽 + 三叉神经痛　；参考：《生理学报》2017年01期

【摘要】：三叉神经痛(trigeminal neuralgia,TN)是局限于三叉神经1支或多支分布区域的反复发作的短暂剧烈疼痛,临床发病率高,严重影响患者生活质量,难以完全治愈。本研究旨在探讨川芎嗪(tetramethylpyrazine,TMP)对慢性压迫性损伤眶下神经(chronic constriction injury of the infraorbital nerve,ION-CCI)致大鼠TN的影响。Sprague-Dawley大鼠随机分为4组:假手术组(sham)、假手术+川芎嗪组(sham+TMP)、模型组(TN)、模型+川芎嗪组(TN+TMP)。通过ION-CCI建立大鼠TN模型,术后2周每天一次腹腔注射川芎嗪(50 mg/kg)。应用电子测痛仪观察各组大鼠机械痛敏阈值的变化。同时采用RT-PCR、蛋白印迹、免疫组织化学染色等方法检测大鼠术侧三叉神经节(trigeminal ganglia,TGs)中降钙素基因相关肽(calcitonin gene-related peptide,CGRP)的表达变化。术后15天内,TN组大鼠手术侧眶下神经面部感觉区域的机械痛敏阈值和sham组相比明显降低,术后第9天到第15天,TN+TMP组和TN组相比较阈值出现明显升高(P0.05)。术后第15天,RT-PCR、蛋白印迹、免疫组织化学染色结果表明,与sham组相比,TN组大鼠TGs中CGRP表达明显升高(P0.05);与TN组相比,TN+TMP组大鼠TGs中CGRP表达显著降低(P0.05)。以上结果表明,TGs中的CGRP对TN的痛觉传递有着重要作用,TMP对TN有一定的抑制作用,其机制可能和抑制TGs中CGRP表达有关。
[Abstract]:Trigeminal neuralgia (TNN) is a brief and acute pain limited to one or more branches of trigeminal nerve. The clinical incidence is high, which seriously affects the quality of life of patients and is difficult to cure completely. The purpose of this study was to investigate the effects of tetramethylpyrazine (TMP) on TN in rats with chronic constriction injury of the infraorbital nervetitis induced by chronic compression injury. Sprague-Dawley rats were randomly divided into four groups: sham-operated group, sham group, model group TNT group, model group TMP group. The TN model of rats was established by ION-CCI and 50 mg / kg of ligustrazine was injected intraperitoneally once a day 2 weeks after operation. The changes of mechanical pain sensitivity threshold in each group were observed by electronic pain measuring instrument. The expression of calcitonin gene related peptide (calcitonin gene-related peptidea CGRP) in the trigeminal ganglion of rat was detected by RT-PCR, Western blot and immunohistochemical staining. Compared with the sham group, the mechanical pain sensitivity threshold of the TN group was significantly lower than that of the sham group within 15 days after operation, and the threshold value of TN TMP group was significantly higher than that of the TN group from the 9th to the 15th day after operation compared with the TN group. On the 15th day after operation, the results of RT-PCR, Western blot and immunohistochemical staining showed that the expression of CGRP in TGs of TN group was significantly higher than that of sham group, and the expression of CGRP in TGs of TN TMP group was significantly lower than that of TN group. These results suggest that CGRP in TGs plays an important role in the transmission of TN pain. TMP may inhibit the expression of CGRP in TGs, and its mechanism may be related to the inhibition of CGRP expression in TGs.
【作者单位】：南昌大学基础医学院生理学教研室;南昌大学附属口腔医院预防科;南昌大学玛丽女王学院;
【基金】：supported by grants from the National Natural Science Foundation of China(No.81100829,81360136 and 81460106) Major Disciplines of Academic and Technical Leaders Project of Jiangxi Province,China(No.2014-47) Technology Support Plan of Jiangxi Province,China(No.20142BBG70051)
【分类号】：R745.11

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