Let-7c-1对戊四氮致痫大鼠学习记忆功能的作用

发布时间：2018-06-13 05:48

本文选题：癫痫 + 学习记忆功能　；参考：《中国神经精神疾病杂志》2017年04期

【摘要】：目的研究let-7c-1对戊四氮(pentylenetetrazol,PTZ)致痫大鼠学习记忆功能的影响,探讨其可能机制。方法通过PTZ腹腔注射SD雄性大鼠建立慢性癫痫模型,随机分为癫痫组、干预对照组、let-7c-1激动剂组,各组12只,另设12只大鼠为正常组。28 d后,观察各组大鼠的行为学变化,let-7c-1基因表达及大鼠海马组织中Bcl-2、Caspase3蛋白的表达。结果与正常组相比,癫痫组大鼠逃避潜伏期延长、穿越平台次数减少、在目标象限的总路程缩短,差异有统计学意义(P0.05);癫痫组与干预对照组相比,逃避潜伏期、穿越平台次数、在目标象限的总路程无统计学差异(P0.05);与干预对照组相比,let-7c-1激动剂组逃避潜伏期明显延长,穿越平台次数减少、在目标象限的总路程缩短,差异有统计学意义(P0.05)。干预对照组与let-7c-1激动剂组let-7c-1基因相对表达量分别为(1.35±0.32)、(62.53±21.01)(F=50.97,P0.05)。癫痫组let-7c-1基因表达量高于正常组,差异有统计学意义(P0.05)。let-7c-1激动剂组let-7c-1基因表达量明显高于干预对照组、癫痫组及正常组,差异有统计学意义(P0.05)。与正常组相比,癫痫组的Bcl-2蛋白表达减少,Caspase3蛋白表达增加,差异有统计学意义(P0.05);癫痫组与干预对照组相比,Bcl-2蛋白及Caspase3蛋白的表达无统计学差异(P0.05)。与干预对照组相比,let-7c-1激动剂组Bcl-2蛋白表达明显减少,Caspase3蛋白表达明显增加,差异有统计学意义(P0.05)。结论 Let-7c-1可能通过减少海马组织Bcl-2蛋白及增加Caspase-3蛋白表达使PTZ致痫大鼠的学习记忆功能受损。
[Abstract]:Objective to study the effect of let-7c-1 on learning and memory function in pentylenetetrazolium PTZs rats and to explore its possible mechanism. Methods chronic epilepsy model was established by intraperitoneal injection of PTZ in SD male rats. The rats were randomly divided into epilepsy group (n = 12), intervention control group (n = 12), let-7c-1 agonist group (n = 12) and control group (n = 12). The expression of let-7c-1 gene and the expression of Bcl-2Caspase3 protein in hippocampus of rats were observed. Results compared with the normal group, the escape latency of the epileptic rats was prolonged, the times of crossing the platform decreased, the total distance in the target quadrant was shortened, the difference was statistically significant (P0.05), the Epilepsy group compared with the intervention control group, the escape latency, the times of crossing the platform, Compared with the intervention control group, the escape latency of the let-7c-1 agonist group was significantly prolonged, the times of crossing the platform decreased, and the total distance in the target quadrant was shortened, the difference was statistically significant (P 0.05). The relative expression of let-7c-1 gene in the intervention control group and the let-7c-1 agonist group was 62.53 卤21.01, respectively. The expression of let-7c-1 gene in epileptic group was higher than that in normal group, and the difference was statistically significant. The expression of let-7c-1 gene in epileptic group was significantly higher than that in intervention control group, and the difference was statistically significant in epileptic group and normal group (P 0.05). Compared with the control group, the expression of Bcl-2 protein in epileptic group decreased and the expression of Caspase3 protein increased, the difference was statistically significant (P0.05), and there was no significant difference in the expression of Bcl-2 protein and Caspase3 protein between epileptic group and intervention control group (P0.05). Compared with the intervention control group, the expression of Bcl-2 protein in the let-7c-1 agonist group was significantly decreased, and the expression of Caspase3 protein was significantly increased. The difference was statistically significant (P 0.05). Conclusion Let-7c-1 may impair the learning and memory function of PTZ induced epilepsy rats by decreasing Bcl-2 protein and increasing Caspase-3 protein expression in hippocampus.
【作者单位】：广西医科大学第一附属医院神经内科;广西壮族自治区人民医院康复医学科;广西贵港市人民医院神经内科;
【基金】：国家自然科学基金(编号:81360201)
【分类号】：R742.1

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