血浆miR-27b、miR-10b与脑动脉粥样硬化及脂代谢异常的关系

发布时间：2018-06-17 04:54

本文选题：miRNA-27b + miRNA-10b　；参考：《青岛大学》2017年硕士论文

【摘要】：目的:探讨血浆miR-27b、miR-10b与脑动脉粥样硬化及脂代谢异常的关系。方法:选取47例同期健康查体者(对照组)、38例无症状脑动脉粥样硬化患者(AS组)、52例大动脉粥样硬化性脑梗死患者(LAA组)和52例小动脉闭塞性脑梗死患者(SAO组),实时荧光定量PCR(qRT-PCR)检测血浆中miR-27b和miR-10b的表达水平;为评价脑动脉粥样硬化病变广泛程度,将对照组设为非动脉粥样硬化组,LAA组及AS组设为动脉粥样硬化组,将动脉粥样硬化组按照单支、双支、多支(三支及以上)病变分为3个亚组,Spearman相关分析miR-27b和miR-1Ob的表达水平与脑动脉粥样硬化血管病变广泛程度的相关性;应用Pearson相关分析血浆miR-27b和miR-10b的表达水平与血脂之间的相关性;应用受试者工作特征(ROC)曲线评估miR-27b和miR-10b对脑动脉粥样硬化识别的准确性。结果:miR-27b、miR-10b和TC水平在动脉粥样硬化组表达水平显著高于对照组及 SAO 组(P0.05),而在对照组与 SAO 组(P0.05)、AS 组与 LAA 组(P0.05)之间差异均无统计学意义,提示miR-27b、miR-10b与脑动脉粥样硬化有关,且梗死对其影响较小;Spearman相关分析,结果显示miR-27b及miR-10b其血浆表达水平与脑动脉粥样硬化血管病变广泛程度有相关性(r=-0.571,P0.001;r=-0.521,P0.001)。Pearson相关分析,结果显示miR-27b及miR-10b的血浆表达水平在动脉粥样硬化组及非动脉粥样硬化组中均与TC水平均有相关性(r动27=-0.859,P动270.001;r 动 10=-0.594,P 动 100.001;r 非 27=-0.426,P 非 27=0.003;r 非 10=-0.653,P非100.001)。利用ROC曲线评估miR-27b和miR-10b对脑动脉粥样硬化的识别效能,ROC 曲线下面积分别为 0.932(95%CI:0.884-0.980,P0.001);0.924(95%CI:0.870-0.979,P0.001),cut-off 值为 5.48;5.82,灵敏度和特异度分别为 89.4%、91.1%;85.1%、96.7%。结论:miR-27b及miR-10b的血浆表达水平在AS组及LAA组中较对照组及SAO组明显上调,但在AS组与LAA组、对照组与SAO组之间无明显统计学差异,而且,miR-27b及miR-10b在血浆中的表达水平与脑动脉粥样硬化广泛程度具有明显相关。这说明,miR-27b及miR-10b不仅与脑动脉粥样硬化有关,并且当miR-27b及miR-10b在血浆中表达越高时,脑动脉粥样硬化的病变越广泛。
[Abstract]:Aim: to investigate the relationship between miR-27b miR-10b and cerebral atherosclerosis and abnormal lipid metabolism. Methods: 47 healthy examiners (38 asymptomatic cerebral atherosclerosis patients in the control group) and 52 patients with arteriosclerotic cerebral infarction (ACI group) and 52 small artery occlusive cerebral infarction patients (SAO group) were selected. The expression levels of miR-27b and miR-10b in plasma were detected by PCR qRT-PCR. In order to evaluate the extent of cerebral atherosclerosis, the control group was divided into non-atherosclerosis group (LAA) and as group (group A) as atherosclerosis group, and atherosclerosis group was divided into two groups according to single and double branches. Spearman correlation Analysis between the expression of miR-27b and miR-1Ob and the extent of atherosclerotic vascular lesions; Pearson correlation was used to analyze the correlation between the expression of miR-27b and miR-10b in plasma and serum lipid, and the accuracy of miR-27b and miR-10b in the recognition of cerebral atherosclerosis was evaluated by ROC- curve. Results the expression levels of miR-27b miR-10b and TC in atherosclerosis group were significantly higher than those in control group and SAO group (P 0.05), but there was no significant difference between control group and SAO group (P 0.05), suggesting that miR-27b miR-10b was associated with cerebral artery atherosclerosis. The results of Spearman correlation analysis showed that the plasma expression levels of miR-27b and miR-10b were correlated with the extent of atherosclerotic vascular lesions. The results showed that the plasma levels of miR-27b and miR-10b were correlated with TC levels in atherosclerosis group and non-atherosclerotic group. The plasma levels of miR-27b and miR-10b were correlated with TC level in atherosclerotic group and non-atherosclerosis group. The plasma levels of miR-27b and miR-10b were correlated with TC level in atherosclerotic group and non-atherosclerosis group. The plasma levels of miR-27b and miR-10b were correlated with TC level in atherosclerotic group and non-atherosclerosis group. The plasma levels of miR-27b and miR-10b were correlated with TC level in atherosclerotic group and non-atherosclerosis group. The ROC curve was used to evaluate the recognition effectiveness of miR-27b and miR-10b for atherosclerosis. The area under the ROC curve was 0.932 ~ 95% CI: 0.884-0.980% (P 0.001), and the cut-off value was 5.485.82, and the sensitivity and specificity were 89.44 ~ 91.1% and 96.771, respectively. Conclusion the plasma levels of miR-27b and miR-10b in as group and LAA group were significantly higher than those in control group and SAO group, but there was no significant difference between as group and LAA group, control group and SAO group. The expression levels of miR-27b and miR-10b in plasma were significantly correlated with the extent of cerebral atherosclerosis. These results suggest that miR-27b and miR-10b are not only associated with atherosclerosis, but also that the higher the expression of miR-27b and miR-10b in plasma, the wider the lesion of cerebral atherosclerosis.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.3

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