支配牙髓的三叉神经分支神经胞体在三叉神经节内的区域分布

发布时间：2018-06-18 21:13

本文选题：三叉神经节 + 三叉神经痛　；参考：《安徽医科大学》2014年硕士论文

【摘要】：目的观察正常及三叉神经疼痛模型大鼠支配牙髓的三叉神经分支的神经胞体在三叉神经节内的区域分布情况，了解正常及三叉神经痛模型大鼠牙髓神经胞体在三叉神经节内与被支配牙髓的定位关系，为临床通过三叉神经节处理治疗三叉神经痛提供解剖学证据。方法通过在正常大鼠（假手术组）以及眶下神经慢性缩窄性损伤（三叉神经痛模型）大鼠（模型组）的不同牙髓腔内（左侧上中切牙牙髓、右侧下中切牙牙髓、左侧上中切牙牙髓和左侧下颌第一磨牙牙髓）注入逆行荧光探针（Fast DiO和/或Fast DiI），72h后用荧光显微镜观察各组大鼠三叉神经节中的被荧光标记的神经胞体分布情况。结果正常大鼠的右下侧中切牙注入荧光探针Fast DiO72h后，，在其同侧三叉神经节的后外侧区域可以观察到有少量绿色荧光标记的神经元，约3-5个，成簇分布。正常大鼠的左上侧中切牙注入荧光探针Fast DiO72h后，在其同侧三叉神经节的中间区域段可以观察到绿色荧光标记的神经元，约数个，也成簇分布。正常大鼠右侧下中切牙及左侧上中切牙髓腔分别注射荧光探针Fast DiO和Fast DiI，72h后发现在其右侧三叉神经节的后外侧区域及左侧三叉神经节的中间区域可分别观察到标记的神经元。正常大鼠左侧上中切牙及左侧下颌第一磨牙髓腔分别注射荧光探针Fast DiI和Fast DiO，72h后发现在其左侧三叉神经节的中后段区域及后外侧区域可观察到少量成簇分布的红色和绿色荧光标记的神经元。以上所有实验观察在其对侧三叉神经节相应位置以及其它部位均未见有荧光标记的神经元。在三叉神经疼痛模型大鼠的相应的牙髓腔内注入相应的荧光标记物后，在三叉神经节内检测到带有荧光的神经元分布及荧光数量和强度没有明显的变化。结论支配牙髓的三叉神经的各分支胞体在三叉神经节内的分布具有一定的区域性，且为单侧性投射；三叉神经痛发生后并不影响三叉神经节内神经元的数量以及投射联系，三叉神经痛的发生可能与三叉神经元的功能活动改变有关。
[Abstract]:Objective to observe the distribution of nerve cell bodies in trigeminal ganglion of normal and trigeminal pain rats. Objective to investigate the relationship between the localization of dental pulp neuronal bodies in the trigeminal ganglion and the innervated pulp in normal and trigeminal neuralgia rats, and to provide anatomic evidence for the treatment of trigeminal neuralgia through the treatment of trigeminal ganglion. Methods the pulp of left superior incisor and right inferior incisor of rats with chronic constrictive injury of suborbital nerve (trigeminal neuralgia model) were studied in normal rats (sham-operated group) and rats (model group). The retrograde fluorescent probe Fast Dio and / or Fast DiI were injected into the pulp of left central incisor and left mandibular first molar for 72 hours. The distribution of fluorescent labeled neuronal bodies in the trigeminal ganglion of rats in each group was observed by fluorescence microscope. Results in the ipsilateral trigeminal ganglion, a small number of green fluorescent labeled neurons were observed in the ipsilateral trigeminal ganglion, about 3-5 neurons in clusters, after the injection of fluorescent probe Fast Dio into the right lower central incisor of normal rats. Green fluorescent labeled neurons were observed in the middle region of the ipsilateral trigeminal ganglion after fluorescent probe Fast Dio was injected into the left upper incisor of normal rats. The labeled neurons were observed in the posterolateral region of the right trigeminal ganglion and the middle region of the left trigeminal ganglion in the right inferior incisor and the left superior incisor respectively 72 h after the injection of the fluorescent probes Fast Dio and Fast DiI respectively into the pulp cavity of the right inferior incisor and the left superior incisor of the normal rat. Fluorescence probes Fast Dii and Fast Dio were injected into the pulp cavity of left upper incisor and left mandibular first molar in normal rats for 72 hours, respectively. A few clusters of red were observed in the middle and posterior segment of left trigeminal ganglion and posterolateral region of left trigeminal ganglia. Colored and green fluorescent labeled neurons. No fluorescent labeled neurons were found in the contralateral trigeminal ganglion. The distribution of fluorescent neurons, the quantity and intensity of fluorescence in trigeminal ganglion were not significantly changed after the corresponding fluorescent markers were injected into the dental pulp cavity of the rats with trigeminal nerve pain. Conclusion the distribution of the branches of trigeminal nerve in the trigeminal ganglion is regional and unilateral, and the number of neurons in trigeminal ganglion is not affected after the occurrence of trigeminal neuralgia. The occurrence of trigeminal neuralgia may be related to the functional changes of trigeminal neurons.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R745.11

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