去乙酰化酶3在人颈动脉粥样硬化斑块中的表达及其临床意义

发布时间：2018-06-20 01:17

  本文选题：动脉粥样硬化 + 去乙酰化酶　； 参考：《中风与神经疾病杂志》2017年12期

【摘要】：目的检测人颈动脉粥样硬化斑块组织及正常颈动脉组织中去乙酰化酶3(SIRT3)的表达水平,分析其与颈动脉粥样硬化斑块形成的关系及临床意义。方法利用HE染色、Masson’s trichrome染色及油红O染色对6例颈动脉组织进行粥样硬化斑块分期,利用免疫组化法和Western blot检测不同分期的斑块组织及正常颈动脉组织中SIRT3的蛋白表达差异,利用免疫荧光共定位法观察SIRT3的细胞定位。结果 6例组织分别为2例正常血管组织,2例Ⅰ期动脉粥样硬化组织,2例Ⅳ期动脉粥样硬化斑块组织,Western blot及免疫组化染色显示SIRT3在Ⅳ期颈动脉粥样硬化斑块组织中显著表达,正常血管组织及Ⅰ期粥样硬化血管组织中未见SIRT3表达;免疫荧光共定位染色结果显示,SIRT3在Ⅳ期斑块内的内皮细胞、平滑肌细胞和巨噬细胞内均有显著表达。结论SIRT3在颈动脉粥样硬化Ⅳ期斑块中表达显著增高,可能作为评估粥样硬化稳定性的一个重要指标。
[Abstract]:Objective to detect the expression of deacetylase 3 (SIRT3) in human carotid atherosclerotic plaque and normal carotid artery, and to analyze its relationship with carotid atherosclerotic plaque formation and its clinical significance. Methods six cases of carotid atherosclerotic plaques were staging with HE staining and oil red O staining. The protein expression of SIRT3 was detected by immunohistochemistry and Western blot. The localization of SIRT3 cells was observed by immunofluorescence co-localization. Results the expression of SIRT3 in carotid atherosclerotic plaques at stage 鈪，

本文编号：2042233