高频电刺激小鼠坐骨神经促进骨骼肌自噬

发布时间：2018-06-24 09:05

本文选题：电刺激 + 骨骼肌　；参考：《生理学报》2017年04期

【摘要】：本研究旨在探讨运动对骨骼肌自噬的调控作用。以小鼠为研究对象,通过高频(100 Hz)电刺激单侧坐骨神经的方法使同侧骨骼肌收缩,以对侧肌肉作为未刺激对照组。分别收集电刺激完成后0、30和60 min的腓肠肌肌肉组织,迅速投入液氮冷冻备用。采用实时荧光定量PCR和Western blot检测自噬相关基因mRNA与蛋白的表达水平。研究结果显示,在电刺激完成后0 min,相比对侧未刺激骨骼肌,电刺激一侧的骨骼肌腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)活性升高,自噬标志蛋白LC3-II/I比率显著升高,蛋白酶体泛素连接酶基因(atrogin-1和MuRF-1)和自噬相关基因(CathepsinL、Bnip3和Bnip3l)mRNA表达均显著上调,自噬相关蛋白ULK1活性升高。上述结果提示,电刺激引起的骨骼肌收缩可促进骨骼肌自噬水平升高,该过程可能通过AMPK/ULK1介导的信号途径调控。
[Abstract]:The aim of this study was to investigate the effects of exercise on skeletal muscle autophagy. The ipsilateral skeletal muscle was contracted by high frequency (100 Hz) electric stimulation of unilateral sciatic nerve in mice, and contralateral muscle was used as unstimulated control group. The muscle tissues of gastrocnemius were collected for 30 and 60 min after electrical stimulation, and cryopreserved with liquid nitrogen. The mRNA and protein levels of autophagy related genes were detected by real-time fluorescent quantitative PCR and Western blot. The results showed that the activity of AMP-activated protein kinase (AMP-activated protein kinase) and the ratio of autophagy marker protein LC3-III increased significantly at 0 min after electrical stimulation compared with the contralateral unstimulated skeletal muscle. The expression of proteasome ubiquitin ligase gene (atrogin-1 and MuRF-1) and autophagy associated gene (Cathepsin Lnip3 and Bnip3l) mRNA were significantly up-regulated and the activity of autophagy associated protein ULK1 was increased. These results suggest that the contraction of skeletal muscle induced by electrical stimulation can promote the increase of skeletal muscle autophagy which may be regulated by AMPK / ULK1 mediated signal pathway.
【作者单位】：山西大学生命科学学院;爱因斯坦医学院医学与分子药理学系;
【基金】：supported by grants from the National Institutes of Health,USA(No.DK020541) Shanxi University,Shanxi Province,China(No.113545023)
【分类号】：R746.4

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