HMGB1及TLR2在多发性硬化患者外周血单核细胞中的表达及其作用

发布时间：2018-06-25 01:08

本文选题：多发性硬化 + HMGB　；参考：《现代免疫学》2017年03期

【摘要】：通过检测高迁移率族蛋白B1(high mobility group protein B1,HMGB1)及Toll样受体2(toll like receptor 2,TLR2)在多发性硬化(multiple sclerosis,MS)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达变化及血清中单核细胞趋化蛋白1(monocyte chemotactic protein 1,MCP-1)、IL-17分泌变化,初步探讨HMGB1及TLR2在MS发病中可能的免疫学作用。应用流式细胞术检测MS患者组、健康对照组人群PBMC中HMGB1及TLR2蛋白的相对表达并采用Pearson相关分析观察两者表达的相关性;应用ELISA法检测两组人群血清中MCP-1、IL-17分泌水平。MS组HMGB1、TLR2蛋白的相对表达及MCP-1、IL-17分泌水平较对照组明显上调(P0.01),HMGB1蛋白与TLR2蛋白表达呈正相关(r=0.893,P0.01)。HMGB1可能会通过其受体TLR2启动下游炎性信号传导通路,参与MS免疫损伤过程。
[Abstract]:The expression of high mobility group protein B1 (high mobility group protein B1hMGB1 and Toll-like receptor 2 (toll like receptor 2TLR2 in peripheral blood mononuclear cells (peripheral blood mononuclear cells) of patients with multiple sclerosis (multiple sclerosis MS) and the secretion of IL-17 in serum monocyte chemoattractant protein 1 (monocyte chemotactic protein 1 / MCP-1 were detected. To explore the possible immunological role of HMGB1 and TLR2 in the pathogenesis of MS. The relative expression of HMGB1 and TLR2 in PBMC of MS patients and healthy controls was detected by flow cytometry and the correlation between HMGB1 and TLR2 was observed by Pearson correlation analysis. The relative expression of HMGB1 / TLR2 protein in serum of two groups of people was detected by Elisa. The relative expression of HMGB1 / TLR2 protein in MS group was significantly higher than that in control group (P0.01). There was a positive correlation between HMGB1 protein and TLR2 protein expression (rn 0.893, P0.01). HMGB1 might activate downstream inflammation through its receptor TLR2. Sex signal transduction pathway, To participate in the process of MS immune injury.
【作者单位】：宁夏医科大学;宁夏回族自治区第三人民医院;宁夏医科大学基础医学院;
【基金】：2015年宁夏医科大学校级课题(XM2015081)
【分类号】：R744.51

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