不同剂量重组人粒细胞集落刺激因子对大鼠脑缺血再灌注损伤的保护作用

发布时间：2018-06-26 06:34

本文选题：再灌注损伤 + 粒细胞集落刺激因子　；参考：《中华临床医师杂志(电子版)》2016年06期

【摘要】：目的探讨重组人粒细胞集落刺激因子(rh G-CSF)对大鼠脑缺血再灌注损伤的神经保护机制并揭示其量效关系。方法 60只雄性SD大鼠随机分为假手术组、模型组、rh G-CSF低、中、高剂量组,采用longa线栓法制作大鼠脑缺血再灌注模型,治疗组于大鼠脑缺血2 h再灌注即刻及24 h分别腹部皮下注射rh G-CSF 25μg/kg、50μg/kg及100μg/kg,假手术组与模型组给予等量生理盐水。术后24 h采用longa及Berderson标准评分法行神经功能评分,2,3,5-氯化三苯基四氮唑(TTC)染色法测定脑梗死体积,免疫组化法(SABC)检测大鼠脑组织中Caveolin-1、COX-2的表达情况,HE染色观察脑组织形态并在高倍镜下计算涂片中炎症细胞数反映脑组织炎症反应。结果 (1)模型组可见明显的神经功能缺损、脑梗死灶及炎症细胞浸润;与模型组相比,rh G-CSF中、高剂量组神经功能评分、炎症细胞数明显减少,梗死体积明显减小(P0.01);rh G-CSF低剂量组与模型组比较无统计学意义(P0.05)。(2)与假手术组相比,模型组Caveolin-1、COX-2表达明显增加;与模型组比较,rh G-CSF中、高剂量组Caveolin-1明显升高,COX-2明显降低(P0.01),低剂量组Caveolin-1及COX-2表达无统计学意义(P0.05)。中高剂量组之间在神经功能缺损、梗死体积、炎症细胞数、Caveolin-1、COX-2表达方面比较,差异均无统计学意义(P0.05)。结论 rh G-CSF可能通过升高Caveolin-1,进而降低COX-2表达,对脑缺血再灌注损伤产生神经保护作用,中剂量50μg/kg可能为最佳应用剂量。
[Abstract]:Objective to investigate the neuroprotective mechanism of recombinant human granulocyte colony stimulating factor (rh G-CSF) on cerebral ischemia-reperfusion injury in rats and reveal its dose-effect relationship. Methods Sixty male Sprague-Dawley rats were randomly divided into sham-operated group, model group with low, middle and high doses of rh G-CSF, and the model of cerebral ischemia-reperfusion was made by longa method. The rats in the treatment group were subcutaneously injected with rh G-CSF 25 渭 g / kg ~ (50 渭 g/kg) and 100 渭 g 路kg ~ (-1) 路kg ~ (-1) at 2 h and 24 h after cerebral ischemia, respectively. The sham operation group was given the same amount of normal saline as the model group. Longa and Berderson standard scores were used to measure the volume of cerebral infarction 24 hours after operation. Immunohistochemical method (SABC) was used to detect the expression of Caveolin-1 COX-2 in rat brain. The morphology of brain tissue was observed by HE staining and the number of inflammatory cells in the smear was calculated under high power microscope to reflect the inflammatory response of brain tissue. Results (1) in the model group, there were obvious neurological function defects, cerebral infarction and inflammatory cell infiltration, and the number of inflammatory cells in the high dose group was significantly lower than that in the rh G-CSF group. There was no significant difference in infarct volume between low dose group and model group (P0.05). (2). Compared with sham operation group, the expression of Caveolin-1 COX-2 in model group was significantly higher than that in model group, and the expression of COX-2 in rh G-CSF in model group was significantly higher than that in model group. Caveolin-1 increased significantly in high dose group (P0.01), while Caveolin-1 and COX-2 expression in low dose group was not significant (P0.05). There was no significant difference in the expression of Caveolin-1 COX-2 between the middle and high dose groups in nerve function defect, infarct volume and inflammatory cell number (P0.05). Conclusion rh G-CSF may decrease the expression of COX-2 by increasing Caveolin-1, which may have a neuroprotective effect on cerebral ischemia-reperfusion injury. The middle dose of 50 渭 g/kg may be the best dose.
【作者单位】：山西医科大学第一医院神经内科;
【分类号】：R743

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