大麻素Ⅱ型受体激动剂HU-308对乙醇所致小胶质细胞分泌IL-6的影响

发布时间：2018-06-26 22:42

本文选题：HU-308 + CB2受体激动剂　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的用不同浓度的乙醇干预小胶质细胞，选择出可以使小胶质细胞损伤又不至于大量致死的乙醇浓度；探讨大麻素II型受体激动剂HU-308对乙醇所致小胶质细胞分泌白细胞介素-6（IL-6）的影响。方法以离体小鼠的小胶质细胞作为研究对象，，分别用(0.00％，0.025％，0.050％，0.1％，0.2％)浓度的乙醇干预小胶质细胞72小时，用酶联免疫吸附法（Elisa法）检测IL-6水平。经过实验、统计学分析，选择0.050％浓度的乙醇用于后续实验。将六孔板中离体的小鼠小胶质细胞培养至80％满瓶底时，分别加入0.050％浓度的乙醇干预，混匀。再将其随机的分为对照组、1umol/LHU-308干预组、5umol/LHU-308干预组、10umol/LHU-308干预组。依次用1umol/L、5umol/L、10umol/LHU-308干预72小时。用Elisa法检测IL-6水平。经过试验、统计学分析，得出结论。结果 0.00％乙醇组与0.025％乙醇组比较差别无统计学意义，与0.050％乙醇组、0.10％乙醇组比较差别均有统计学意义，0.025％乙醇组与0.050％乙醇组、0.10％乙醇组比较差别均有统计学意义，0.050％乙醇组与0.10％乙醇组比较差别无统计学意义。对照组、1umol/LHU-308干预组、5umol/LHU-308干预组、10umol/LHU-308干预组IL-6浓度的均数值依次降低。对照组与1umol/LHU-308干预组、5umol/LHU-308干预组、10umol/LHU-308干预组比较差别均有统计学意义，1umol/LHU-308干预组与5umol/LHU-308干预组、10umol/LHU-308干预组比较差别均有统计学意义，5umol/LHU-308干预组与10umol/LHU-308干预组比较差别无统计学意义。结论 0.025％的乙醇对小胶质细胞分泌IL-6水平无明显影响。0.20％的乙醇则使大部分小胶质细胞死亡。0.050％乙醇与0.10％乙醇处理小胶质细胞，IL-6分泌水平无明显差别且均高于对小胶质细胞产生炎性损伤的水平，故选用0.050％乙醇浓度作为后续实验。 HU-308可以降低乙醇所致的小胶质细胞分泌IL-6的水平，且5umol/LHU-308干预与10umol/LHU-308干预无明显差别。
[Abstract]:objective
The effects of II receptor agonist HU-308 on the secretion of interleukin -6 (IL-6) in microglia induced by ethanol were investigated by interfering with different concentrations of ethanol in microglia.
Method
The microglia in isolated mice was used as the study object. The microglia was treated with ethanol (0%, 0.025%, 0.050%, 0.1%, 0.2%) for 72 hours respectively. The IL-6 level was detected by enzyme linked immunosorbent assay (Elisa method). After the experiment and statistical analysis, the ethanol of 0.050% concentration was selected for the follow-up experiment.
When the isolated mouse microglia in the six pore plate was cultured to the bottom of the 80% full bottle, 0.050% concentration of ethanol was added to the control group. Then it was randomly divided into control group, 1umol/LHU-308 intervention group, 5umol/LHU-308 intervention group and 10umol/LHU-308 intervention group. 1umol/L, 5umol /L, 10umol/LHU-308 intervention for 72 hours. IL- was used to detect IL- by Elisa method. The 6 level. Through the test, statistical analysis, draw the conclusion.
Result
There was no significant difference in the difference between the 0% ethanol group and the 0.025% ethanol group. Compared with the 0.050% ethanol group and the 0.10% ethanol group, there were significant differences in the difference between the 0.025% ethanol group and the 0.050% ethanol group and the 0.10% ethanol group. There was no significant difference in the difference between the 0.050% ethanol group and the 0.10% ethanol group.
The control group, the 1umol/LHU-308 intervention group, the 5umol/LHU-308 intervention group and the 10umol/LHU-308 intervention group decreased the IL-6 concentration in turn. The control group and the 1umol/LHU-308 intervention group, the 5umol/LHU-308 intervention group, the 10umol/LHU-308 intervention group had statistical significance, 1umol/LHU-308 intervention group and 5umol/LHU-308 intervention group, 10umol/LHU-308 intervention. There was statistically significant difference between the two groups. There was no significant difference between the 5umol/LHU-308 intervention group and the 10umol/LHU-308 intervention group.
conclusion
0.025% ethanol had no significant effect on the secretion of IL-6 in microglia and.0.20% ethanol, while most microglia died of.0.050% ethanol and 0.10% ethanol to treat microglia. The level of IL-6 secretion was not significantly different and higher than that of microglia. The concentration of 0.050% ethanol was selected as the post. Continue the experiment.
HU-308 could reduce the level of IL-6 secreted by microglia induced by ethanol, and there was no significant difference between 5umol/LHU-308 intervention and 10umol/LHU-308 intervention.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R741

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