脑卒中后抑郁大鼠额前皮质小胶质细胞表达BDNF及其受体TrkB

发布时间：2018-07-01 19:24

  本文选题：脑卒中后抑郁 + 额前皮质　； 参考：《神经解剖学杂志》2017年06期

【摘要】：目的:探讨脑卒中后抑郁(post-stroke depression,PSD)大鼠额前皮质小胶质细胞表达脑源性神经营养因子(BDNF)及其高亲和力受体酪氨酸激酶受体B(TrkB)的情况,了解小胶质细胞在PSD发病机制中的作用。方法:将健康成年SD大鼠随机分为正常组、抑郁组、脑卒中组和PSD组,每组10只。卒中组采用线栓法建立局灶性脑缺血模型;抑郁组采用慢性不可预见的中等应激刺激(CUMS)结合孤养法建立大鼠慢性应激抑郁模型;PSD组采用线栓法建立局灶性脑缺血模型后,再加以CUMS及孤养法建立PSD大鼠模型。于造模后第29 d(4周后)及第57 d(8周后)应用免疫荧光双标染色法检测各组大鼠额前皮质小胶质细胞表达BDNF及其高亲和力受体TrkB的情况。结果:造模后第29 d PSD组额前皮质OX42(小胶质细胞标记物)与BDNF免疫荧光双标阳性细胞及与TrkB免疫荧光双标阳性细胞平均光密度值均最小,正常对照组均最大。单因素方差分析结果显示:PSD组与各对照组(抑郁组、脑卒中组、正常组)相比,差异均有统计学意义(P0.05);抑郁组及脑卒中组均较正常对照组小(P0.05)。造模后第57 d PSD组额前皮质OX42与BDNF免疫荧光双标阳性细胞及OX42与TrkB免疫荧光双标阳性细胞平均光密度值均为最小,正常对照组均最大。PSD组与各对照组(抑郁组、脑卒中组、正常组)相比,差异均有统计学意义(P0.05);抑郁组也较正常对照组小(P0.05);脑卒中组也较正常对照组小(P0.05);抑郁组与脑卒中组相比,差异无统计学意义(P0.05)。结论:PSD大鼠额前皮质小胶质细胞表达BDNF及TrkB,其平均光密度值在PSD急性期及慢性期均明显减少,可能与PSD发病机制相关。小胶质细胞可能通过减少BDNF及其高亲和力受体TrkB的表达在PSD发病过程中发挥了重要的作用。
[Abstract]:Aim: to investigate the expression of brain-derived neurotrophic factor (BDNF) and its high affinity receptor tyrosine kinase B (TrkB) in prefrontal cortex microglia of post-stroke depression- PSD rats, and to investigate the role of microglia in the pathogenesis of post-stroke. Methods: healthy adult SD rats were randomly divided into normal group, depression group, stroke group and PSD group with 10 rats in each group. In stroke group, focal cerebral ischemia model was established by thread occlusion, and chronic unpredictable moderate stress stimulation (CUMS) combined with solitary therapy was used to establish chronic stress depression model in depression group and focal cerebral ischemia model was established in PSD group with thread occlusion method. Then the rat model of PSD was established by CUMS and the method of solitary nourishment. The expression of BDNF and its high affinity receptor TrkB in prefrontal cortex microglia of rats in each group were detected by immunofluorescence double labeling method on the 29th (4 weeks) and 57 (8 weeks) after modeling. Results: the average optical density of prefrontal cortex OX42 (microglial cell marker) and BDNF double labeled positive cells and TrkB immunofluorescence positive cells were the lowest in PSD group on the 29th day after modeling, and were the largest in normal control group. The univariate ANOVA results showed that there were significant differences between the two groups (P 0.05); the depression group and stroke group were smaller than the normal control group (P0.05). The mean optical density of OX42 and BDNF double labeled positive cells and OX42 and TrkB immunofluorescence positive cells in prefrontal cortex of PSD group was the smallest on the 57th day after modeling, and the maximum optical density of OX42 and BDNF double labeled positive cells in PSD group was the highest in the normal control group and every control group (depression group, stroke group). Compared with the normal group, the difference was statistically significant (P0.05); the depression group was also smaller than the normal control group (P0.05); the stroke group was also smaller than the normal control group (P0.05); the depression group and the stroke group, the difference was not statistically significant (P0.05). Conclusion the expression of BDNF and TrkB in microglia of prefrontal cortex of the rats with PSD was significantly decreased in the acute and chronic phase of PSD, which may be related to the pathogenesis of PSD. Microglia may play an important role in the pathogenesis of PSD by reducing the expression of BDNF and its high affinity receptor TrkB.
【作者单位】： 大理大学临床医学院;大理大学第一附属医院神经内科;
【基金】：国家自然科学基金(81360208)
【分类号】：R743.3
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本文编号：2088890