新生小鼠缺氧缺血性脑损伤模型的建立

发布时间：2018-07-05 14:53

  本文选题：缺氧缺血 + 脑损伤　； 参考：《中国实验动物学报》2017年05期

【摘要】：目的对经典Vannucci法进行改进,建立一种简便、稳定的新生小鼠缺血缺氧脑损伤模型。方法将新生11 d的KM小鼠分为正常对照组(N组,n=20)和缺血缺氧组(HIBD组,n=160),对HIBD组行左侧颈总动脉结扎,分别按照C1-C8条件缺氧建模。建模后通过比较各条件下小鼠死亡率、建模成功率和TTC染色脑梗死体积,选取最稳定的建模条件。建模后利用体重生长曲线分析小鼠生长发育情况;Longa、Grip test、悬吊试验评估小鼠神经运动功能;HE染色观察脑组织病理改变。结果新生小鼠行左侧颈总动脉结扎,8% O_2、35℃条件下缺氧45min,死亡率低(8.3%)且成模率高(47.92%);HIBD组较N组小鼠体重增长缓慢并出现严重的神经运动功能障碍;结扎侧出现脑梗死区,约占全脑体积(17.76±0.70)%;结扎侧大脑皮层及海马区神经元出现变性坏死。结论本实验采用新生小鼠行左侧颈总动脉结扎,在8%O2、35℃条件下缺氧45 min复制HIBD动物模型,简便且稳定性好,是用于新生儿缺血缺氧性脑损伤研究较为理想的动物模型。
[Abstract]:Objective to improve the classical Vannucci method and to establish a simple and stable model of cerebral ischemia and hypoxia injury in newborn mice. Methods the km mice were divided into two groups: normal control group (N group) and ischemic hypoxia group (HIBD group). The left common carotid artery was ligated in HIBD group, and the hypoxia model was established according to C1-C8 condition. After modeling, the most stable modeling conditions were selected by comparing the mortality of mice, the success rate of modeling and the volume of cerebral infarction stained by TTC. After modeling, the growth and development of mice were analyzed by weight growth curve, and the neuromotor function of mice was evaluated by suspension test. The pathological changes of brain tissue were observed by HE staining. Results the neonatal mice underwent left common carotid artery ligation (8%) for 45 min under hypoxia, the mortality rate was low (8.3%) and the rate of model formation was higher (47.92%) in HIBD group than that in N group, the body weight of HIBD group increased more slowly than that of N group, and severe neuromotor dysfunction occurred in ligated side, and cerebral infarction area appeared in ligation side. The volume of the whole brain was (17.76 卤0.70) and the neurons in the cortical and hippocampal regions were denatured and necrotized. Conclusion the animal model of HIBD was established by ligation of left common carotid artery in newborn mice under hypoxia for 45 min at 8o 2 鈩，

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