线粒体钙单向转运体在匹鲁卡品致痫大鼠海马神经损伤中的作用

发布时间：2018-07-05 19:48

本文选题：癫痫 + 线粒体钙单向转运体　；参考：《中风与神经疾病杂志》2017年11期

【摘要】：目的研究线粒体钙单向转运体(mitochondrial calcium uniporter,MCU)在氯化锂-匹鲁卡品(pilocarpine,PILO)癫痫大鼠模型神经损伤中的作用。方法将84只雄性SD大鼠随机分成空白对照(CON)组、PILO组(2 h、8 h、24 h和72 h 4个亚组)、Ru360组和精胺(Spermine)组。观察各组大鼠行为学改变,并荧光染色法测定海马组织线粒体Ca~(2+)浓度,TUNEL染色检测海马CA3区神经元凋亡,并采用Western Blot法检测凋亡相关蛋白Bax、Bcl-2、Cyt C和caspase-3表达变化。结果 (1)与CON组比较,PILO组海马神经元线粒体Ca~(2+)浓度明显升高,凋亡明显增加(P0.05);与CON组比较,PILO组海马Bax表达水平、线粒体Cyt C释放及caspase-3激活明显增多,而Bcl-2表达水平明显减少(P0.05)。(2)与PILO组比较,Ru360组海马神经元线粒体Ca~(2+)浓度明显降低,凋亡明显减少(P0.05);与PILO组比较,Ru360组海马Bax表达水平、线粒体Cyt C释放及caspase-3激活明显减少,而Bcl-2表达水平明显增多(P0.05)。(3)与PILO组比较,Spermine组海马神经元线粒体Ca~(2+)浓度明显升高,凋亡明显增加(P0.05);与PILO组比较,Spermine组海马Bax表达水平、线粒体Cyt C释放及caspase-3激活明显增多,而Bcl-2表达水平明显减少(P0.05)。结论 MCU在PILO癫痫大鼠海马神经损伤中发挥重要作用,抑制MCU可发挥对海马神经元凋亡的保护作用,其机制可能是通过抑制线粒体介导的细胞凋亡途径。
[Abstract]:Objective to study the role of mitochondrial calcium one-way transporter (mitochondrial calcium Uniporterus in nerve injury of pilocarpine Pilo epileptic rats. Methods Eighty-four male Sprague-Dawley rats were randomly divided into two groups: control group (control group), Pilo group (2 h 8 h, 24 h and 72 h), Ru360 group and Spermine group. The behavioral changes were observed, and the apoptosis of neurons in hippocampal CA3 area was detected by Tunel staining, and the expression of apoptosis-related protein Bax-2Cyt C and caspase-3 were detected by Western Blot. Results (1) compared with the Con group, the concentration of Ca ~ (2) and apoptosis of hippocampal neurons in the Pilo group increased significantly (P0.05), and the expression of Bax, the release of Cyt C and the activation of caspase-3 in the hippocampus of the Pilo group were significantly increased compared with the control group (P0.05). Compared with Pilo group, the expression of Bcl-2 was significantly decreased (P0.05 /). (_ 2) and the mitochondrial Ca ~ (2) concentration and apoptosis of hippocampal neurons in Ru360 group were significantly decreased (P0.05), and the expression level of Bax in hippocampus of Ru360 group was significantly lower than that of Pilo group (P0.05), and the release of mitochondrial Cyt C and the activation of caspase-3 were significantly decreased in Ru360 group compared with Pilo group. The expression level of Bcl-2 was significantly increased (P0.05). (3) compared with Pilo group, the mitochondrial Ca ~ (2) concentration and apoptosis of hippocampus neurons in Spermine group were significantly increased (P0.05), and the expression level of Bax in hippocampus of Spermine group was significantly higher than that of Pilo group (P0.05), and the release of mitochondrial Cyt C and activation of caspase-3 were significantly increased in Spermine group. The expression of Bcl-2 was significantly decreased (P0.05). Conclusion MCU plays an important role in hippocampal nerve injury in Pilo epileptic rats. Inhibition of MCU may play a protective role on apoptosis of hippocampal neurons, and its mechanism may be through inhibition of mitochondrial mediated apoptosis pathway.
【作者单位】：郑州大学第一附属医院神经内科;郑州大学第一附属医院检验科;
【基金】：国家自然科学基金面上项目(No.81571260)
【分类号】：R742.1

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