促红细胞生成素对新生大鼠皮层神经元体外缺氧缺糖损伤的保护作用

发布时间：2018-07-07 19:30

本文选题：促红细胞生成素 + 皮层神经元　；参考：《吉林大学》2017年硕士论文

【摘要】：背景:促红细胞生成素(EPO)对脑缺血缺氧性损伤具有显著的神经保护作用。它的作用机制有抗凋亡、抗氧化和抗炎作用以及刺激血管生成和神经发生等。许多动物实验或临床研究的结果存在差异,可能与不同的实验方法包括缺氧持续时间、给药时间、给药剂量和频率等有关。EPO对于脑缺血缺氧性损伤显示巨大的神经保护潜力,因此为更好地实现EPO从实验室到临床的应用,以上的每个因素都必须进行研究及评估。目的:研究rhEPO对脑缺血缺氧性损伤的神经保护作用及其有效剂量和作用时间。方法:分离新生的Wistar大鼠皮层神经细胞进行原代培养,用SP法进行神经元纯度鉴定。神经元培养至7d-11d,随机分为正常对照组、OGD损伤组和rhEPO处理组。对于rhEPO处理组神经元,首先进行OGD损伤,之后给予不同剂量的rhEPO(0.1、1、10、100U/ml)处理;另外,在不同的时间(OGD损伤后0h、24h、48h)给予损伤神经元10U/ml rhEPO处理。观察各组细胞形态学改变,MTT法检测细胞存活率。结果:1、原代培养的皮层神经元阳性率达90%以上,符合实验要求。2、与OGD损伤组比较,rhEPO(0.1、1、10U/ml)处理可增加OGD损伤的皮层神经元的存活率(P0.05),且浓度为10U/ml时rhEPO的保护作用最强(P0.05)。皮层神经元OGD损伤后立即加入rhEPO(10U/ml)保护作用最强(P0.05),损伤后24h、损伤后48h给药没有增加皮层神经元存活率。结论:rhEPO对体外缺氧缺糖损伤的大鼠皮层神经元具有保护作用,rhEPO有效浓度为0.1-10U/ml,且其浓度为10U/ml时保护作用最强;rhEPO在神经元损伤后立即给药保护作用最强。
[Abstract]:Background: Erythropoietin (EPO) has a significant neuroprotective effect on cerebral ischemic hypoxic injury. Its mechanisms include anti-apoptosis, anti-oxidation and anti-inflammation, and stimulation of angiogenesis and neurogenesis. The results of many animal experiments or clinical studies may be related to different experimental methods, such as hypoxia duration, dosage and frequency of administration. EPO shows great neuroprotective potential in cerebral ischemic hypoxic injury. Therefore, in order to better realize the application of EPO from laboratory to clinical, each of the above factors must be studied and evaluated. Aim: to study the neuroprotective effect of rhEPO on cerebral ischemic hypoxic injury and its effective dose and time. Methods: the cortical neurons of newborn Wistar rats were isolated and cultured in primary culture, and the purity of neurons was identified by SP method. Neurons cultured for 7 d to 11 d were randomly divided into normal control group and rhEPO treated group. In rhEPO treated group, OGD injury was first performed, then rhEPO was given at different doses of rhEPO (0.1 ~ 10100U / ml), in addition, 10U / ml rhEPO was given at different time (0 h ~ 24h ~ 48h after OGD injury). Cell viability was measured by MTT assay. Results the positive rate of primary cultured cortical neurons was more than 90%. Compared with OGD group, rhEPO (0.1U / ml) could increase the survival rate of OGD injured cortical neurons (P0.05), and the protective effect of rhEPO was the strongest (P0.05) when the concentration was 10Uml. The protective effect of rhEPO (10U / ml) on cortical neurons was the strongest immediately after OGD injury (P0.05). 24 hours after injury and 48 hours after injury, the survival rate of cortical neurons was not increased by administration of rhEPO (10U / ml). Conclusion the effective concentration of rhEPO is 0.1-10U / ml, and the protective effect of rhEPO is strongest when the concentration is 10U / ml. The protective effect of rhEPO is strongest immediately after neuron injury.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743

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