面神经离断伤后核团微环境中NG2阳性细胞及各类胶质细胞的反应

发布时间：2018-07-08 18:55

本文选题：面神经损伤 + 面神经核　；参考：《解放军医学杂志》2017年03期

【摘要】：目的观察面神经离断伤后核团内NG2阳性细胞及各类胶质细胞的反应,探索面神经损伤后核团内胶质细胞微环境的变化。方法建立大鼠面神经离断伤模型,术后1、2、7、14、28d进行脑干及脊髓取材,采用免疫荧光双标、免疫组化结合Cresyl Violet染色观察面神经核团内胶质细胞及NG2阳性细胞的反应,Western blotting检测NG2蛋白表达的变化。结果面神经离断后,面神经核团内小胶质细胞逐渐对受损神经元形成紧密包绕,星形胶质细胞形成花环状簇拥,NG2蛋白呈现规律的时相性表达,NG2阳性细胞对受损神经元进行了广泛的"突触剥离"。结论各类胶质细胞参与了核团内胶质瘢痕的形成,NG2阳性细胞可隔离受损神经元,使其免于遭受兴奋性输入的潜在伤害。
[Abstract]:Objective to observe the response of NG2 positive cells and glial cells in nucleus after facial nerve injury and to explore the changes of microenvironment of glial cells in nucleus after facial nerve injury. Methods the rat model of facial nerve amputation was established. The brain stem and spinal cord were taken from rats on the 1st and 2nd day after operation, and immunofluorescence double labeling was used. Immunohistochemical staining and Cresyl Violet staining were used to detect the expression of NG2 protein in the facial nucleus. Results after the facial nerve was severed, the microglia in the nucleus of the facial nerve gradually formed a close wrapping on the injured neurons. NG2 positive cells express regularly in astrocytes to form a floral ring surrounded by NG2 protein, and the injured neurons have been extensively "synaptically stripped" by NG2 positive cells. Conclusion all kinds of glial cells participate in the formation of glial scar in nucleus and NG2 positive cells can isolate the injured neurons and protect them from the potential injury of excitatory input.
【作者单位】：第二军医大学长征医院整形外科;
【基金】：国家自然科学基金青年项目(81301054) 上海市自然科学基金青年项目(13ZR1450600) 上海市卫生局青年科研项目(2012Y142)~~
【分类号】：R745

【相似文献】