夹竹桃麻素预处理对急性脑梗死脑组织损伤影响的初步研究

发布时间：2018-07-09 15:37

本文选题：急性脑梗死 + 夹竹桃麻素　；参考：《中国现代药物应用》2015年23期

【摘要】：目的初步研究夹竹桃麻素预处理对急性脑梗死脑组织的损伤影响。方法 45只健康雄性小鼠作为研究对象,随机分成正常组、对照组、研究A组、研究B组、研究C组五组,每组9只。分别予以不同处理,比较各组脑梗死面积百分比,并进行免疫印迹分析。结果研究A、B、C组的脑梗死面积百分比分别为14.01%、19.79%、10.18%,对照组的脑梗死面积百分比为19.80%,与对照组的脑梗死面积百分比比较,研究A、C组的脑梗死面积百分比下降显著,差异有统计学意义(P0.05);免疫印迹分析中正常组小鼠大脑中动脉栓塞后,于0.5、1.5 h时,gp91phox,p67phox,p47phox蛋白表达上调、4 h时蛋白表达下调。经夹竹桃麻素预处理后,仅研究C组的p67phox蛋白表达显著下调。结论夹竹桃麻素主要是通过抗氧化应激以抑制缺血区及再灌注区氧化自由基释放,并稳定血脑屏障,从而缩小脑梗死面积,延缓脑梗死损伤发展。
[Abstract]:Objective to study the effect of diosgenin preconditioning on cerebral tissue injury in patients with acute cerebral infarction. Methods Forty-five healthy male mice were randomly divided into normal group (n = 9), control group (n = 9), study group A (n = 9), study group B (n = 5) and study group C (n = 5). The percentage of cerebral infarction area in each group was compared and analyzed by Western blot. Results the percentage of cerebral infarction area was 14.01% and 19.79% in the control group, and 19.80 in the control group, respectively. Compared with the control group, the percentage of cerebral infarction area in the ANAC group was significantly lower than that in the control group, the percentage of cerebral infarction area in the control group was significantly lower than that in the control group, and the percentage of cerebral infarction area in the control group was significantly lower than that in the control group. The difference was statistically significant (P0.05), and the expression of Gp91phoxP67phoxP47phox was up-regulated at 1.5 h after middle cerebral artery embolization (MCAE) in normal mice by Western blot analysis, and the protein expression was down-regulated at 4 h after embolization. The expression of p67phox protein in group C was significantly down-regulated after pretreatment with diosgenin. Conclusion the mechanism of diosgenin is to inhibit the release of oxidative free radicals in ischemic and reperfusion areas and to stabilize the blood-brain barrier through antioxidant stress, so as to reduce the area of cerebral infarction and delay the development of cerebral infarction injury.
【作者单位】：辽宁省大连市中心医院神经内二科;吉林省通化市人民医院内分泌科;大连市中心医院;
【分类号】：R743.3

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