盐酸法舒地尔对蛛网膜下腔出血大鼠学习记忆功能及海马CA1区自噬的影响

发布时间：2018-07-14 11:53

【摘要】：目的研究盐酸法舒地尔对蛛网膜下腔出血(SAH)大鼠学习记忆及海马CA1区自噬的影响。方法雄性Sprague-Dawley大鼠54只,随机分为假手术组、SAH组、药物组,各18只。颈内动脉刺破法造模,药物组造模成功后予盐酸法舒地尔10 mg/kg腹腔注射,每天1次,假手术组和SAH组腹腔注射等量生理盐水,干预后6 h、24 h、72 h予穿梭箱试验;取海马CA1区组织HE染色观察细胞形态,免疫组织化学染色观察Beclin-1和微管相关蛋白l轻链3-Ⅱ(LC3-Ⅱ)的表达。结果与假手术组相比,SAH组大鼠各时间点穿梭箱试验逃避反应次数减少(P0.05),逃避反应时间延长(P0.05);海马CA1区神经细胞数目减少(P0.05),Beclin-1、LC3-Ⅱ表达增高(P0.05)。与SAH组相比,药物组各时间点逃避反应次数增多(P0.05),逃避反应时间减少(P0.05);海马CA1区神经细胞增多(P0.05),Beclin-1、LC3-Ⅱ表达进一步增多(P0.05)。结论盐酸法舒地尔可改善SAH大鼠学习记忆功能,减少神经细胞损伤,增强海马CA1区自噬。
[Abstract]:Objective to study the effects of fasudil hydrochloride on learning and memory and hippocampal CA1 autophagy in rats with subarachnoid hemorrhage (SAH). Methods 54 male Sprague-Dawley rats were randomly divided into sham-operated group (n = 18) and drug group (n = 18). The internal carotid artery puncture method was used to make the model, and the drug group was injected intraperitoneally with fasudil hydrochloride for 10 mg/kg once a day. The sham operation group and SAH group were injected with the same amount of normal saline intraperitoneally. The shuttle-box test was carried out at 24 hours and 72 hours after 6 hours of intervention. The expression of Beclin-1 and microtubule-associated protein l light chain 3- 鈪，

本文编号：2121562