大鼠脑梗死后交叉性小脑神经机能联系不能的DTI评价

发布时间：2018-07-25 18:13

【摘要】：目的:通过DTI检测大鼠大脑中动脉闭塞(Middle cerebral artery occlusion,MCAO)后梗死核心远隔区域扩散参数的变化情况,并运用免疫组化检测脑组织RGMa蛋白的表达。检测脑梗死核心区域及双侧小脑半球的扩散情况及病理改变,进一步了解交叉性神经机能联系不能(Crossed cerebellar diaschisis,CCD)的相关机制。方法:将所有动物随机分为2组,实验组56只,对照组14只。实验组及对照组大鼠在模型制作成功后1 h、3 h、6 h、9 h、12 h、24 h和72 h分别行MRI动态观察,并用免疫组化法检测大鼠双侧小脑半球RGMa蛋白的表达。结果:实验组大鼠MCAO后各时间点,磁共振成像结果示:双侧小脑半球与正常对照组相比,FA值均降低,12 h降至最低,对侧(右侧)小脑半球较同侧(左侧)小脑半球降低更明显。免疫组化结果示:双侧小脑半球与正常对照组相比,RGMa蛋白表达均升高,至24 h达最高峰,对侧小脑半球较同侧小脑半球升高更明显。结论:磁共振DTI技术结合RGMa蛋白病理学检查可以发现幕上脑梗死后CCD现象,并可以解释CCD发生的相关机制。
[Abstract]:Aim: to detect the changes of diffusion parameters of infarct core distant area after middle cerebral artery occlusion (MCAO) in rats by DTI, and to detect the expression of RGMa protein in brain tissue by immunohistochemistry. The diffusion and pathological changes of the central area and bilateral cerebellar hemispheres of cerebral infarction were detected to further understand the mechanism of (Crossed cerebellar. Methods: all the animals were randomly divided into two groups: the experimental group (n = 56) and the control group (n = 14). The dynamic MRI was observed in the experimental group and the control group at 1 h, 3 h, 6 h, 9 h, 12 h, 24 h and 72 h, respectively. The expression of RGMa protein in bilateral cerebellar hemispheres was detected by immunohistochemical method. Results: at each time point after MCAO, the results of magnetic resonance imaging showed that the FA values of bilateral cerebellar hemispheres decreased to the lowest at 12 h, and the contralateral (right) cerebellar hemisphere was significantly lower than that of the ipsilateral (left) cerebellar hemisphere. The results of immunohistochemistry showed that the expression of RGMa protein in bilateral cerebellar hemisphere was higher than that in normal control group, and reached its peak at 24 h, and that in contralateral cerebellar hemisphere was higher than that in ipsilateral cerebellar hemisphere. Conclusion: Mr DTI combined with pathological examination of RGMa protein can detect the CCD phenomenon after supratentorial cerebral infarction and explain the related mechanism of CCD.
【作者单位】：郑州大学第一附属医院;
【分类号】：R743.33

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