神经生长因子对脑梗死大鼠VEGF、SDF-1的影响及其可能机制

发布时间：2018-08-07 18:33

【摘要】：目的:观察神经生长因子(NGF)对脑梗死大鼠血清血管内皮生长因子(VEGF)、基质细胞衍生因子-1(SDF-1)表达的影响,并探讨其可能的机制。方法:将200~250 g的SD雄性大鼠,采用线栓法制备大鼠的一侧大脑中动脉缺血再灌注损伤模型,通过随机法将大鼠随机分成NGF治疗组、溶剂对照组及NGF+PI3K拮抗剂组,每组12只。所有大鼠分别于造模后第4和7天行神经功能评分,采用酶联免疫吸附法检测大鼠脑梗死后第4天时血清VEGF及SDF-1的水平。结果:在脑梗死后第4和7天时,与溶剂对照组及NGF+PI3K拮抗剂组大鼠比较,NGF治疗组大鼠的神经功能评分较低(均P0.05)。脑梗死后第4天时,NGF治疗组大鼠的血清VEGF及SDF-1水平均高于溶剂对照组、NGF+PI3K拮抗剂组(P0.05)。结论:NGF治疗可改善成年大鼠脑梗死缺损的神经功能,并促进脑梗死大鼠血清VEGF及SDF-1的表达上调,PI3K/AKT信号通路可能参与上述作用的调控。
[Abstract]:Aim: to investigate the effect of nerve growth factor (NGF) on the expression of vascular endothelial growth factor (VEGF), stromal cell derived factor 1 (SDF-1) in the serum of rats with cerebral infarction and to explore its possible mechanism. Methods: the male SD rats with 200g 250g were randomly divided into NGF treatment group, solvent control group and NGF PI3K antagonist group with 12 rats in each group. All the rats were assessed on the 4th and 7th day after the model. The levels of serum VEGF and SDF-1 were measured by enzyme-linked immunosorbent assay (Elisa) on the 4th day after cerebral infarction. Results: on the 4th and 7th day after cerebral infarction, the neurological function scores of rats in NGF PI3K treatment group were lower than those in solvent control group and NGF PI3K antagonist group (P0.05). On the 4th day after cerebral infarction, the levels of serum VEGF and SDF-1 in the NGF treated group were higher than those in the solvent control group (P0.05). Conclusion the treatment of VEGF can improve the neurological function of cerebral infarction in adult rats and promote the expression of VEGF and SDF-1 in the serum of rats with cerebral infarction. The PI3K / AKT signaling pathway may be involved in the regulation of these effects.
【作者单位】：广州市红十字会医院暨南大学医学院附属广州红十字会医院神经内科;南方医科大学深圳医院神经内科;广州市红十字会医院暨南大学医学院附属广州红十字会医院超声诊断科;
【基金】：国家自然科学基金项目(编号:81100898) 广东省自然科学基金项目(编号:2014A030313681)
【分类号】：R743.33

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