Toll样受体4和核因子-κB在颅内动脉瘤组织中的表达及作用机制

发布时间：2018-08-08 13:55

【摘要】：目的探讨Toll样受体(TLR)4和核因子(NF)-κB在颅内动脉瘤组织中的表达及作用机制。方法在开颅动脉瘤夹闭术中收集18例动脉瘤标本,根据Hunt-hess标准分为破裂组10例、未破裂组8例;取开颅夹闭术中获取入路同侧血管10份为正常对照组。分别行光镜和电镜观察标本病理学改变,α-actin染色结合电镜观察计算出血管平滑肌细胞密度;免疫组化法检测标本中TLR4、NF-κB、CD68、半胱氨酸天冬氨酸蛋白酶(Caspase)-3表达水平;RT-PCR和Western印迹检测标本中TLR4、NF-κB mRNA和蛋白的相对表达量。结果动脉瘤血管壁分布有少量阳性平滑肌细胞,而正常血管壁平滑肌细胞α-actin染色几乎均为阳性,其中破裂组、未破裂组的血管平滑肌细胞密度明显低于正常对照组(P0.01);破裂组血管平滑肌细胞密度明显低于未破裂组(P0.05)。破裂组、未破裂组TLR4、NF-κB、CD68、Caspase-3表达水平明显高于正常对照组,破裂组明显高于未破裂组(P0.05)。破裂组、未破裂组TLR4和NF-κB mRNA和蛋白的相对表达量均明显高于正常对照组,破裂组明显高于未破裂组(P0.05)。结论 TLR4/NF-κB信号通路在血管内皮损伤介导的颅内动脉瘤血管壁炎性反应和平滑肌细胞介导的退行性病变过程中发挥重要作用。
[Abstract]:Objective to investigate the expression and mechanism of Toll like receptor (TLR) 4 and nuclear factor (NF)-魏 B in intracranial aneurysms. Methods 18 cases of aneurysms were collected and divided into rupture group (n = 10) and unruptured group (n = 8) according to Hunt-hess criteria. The pathological changes were observed by light microscope and electron microscope, and the density of vascular smooth muscle cells was calculated by 伪 -actin staining combined with electron microscope. The expression level of (Caspase)-3 of cysteine aspartate protease (Caspase)-3) was detected by immunohistochemistry. The relative expression of TLR4 NF- 魏 B mRNA and protein was detected by RT-PCR and Western blot. Results A small number of positive smooth muscle cells were found in aneurysm wall, but 伪 -actin staining was almost positive in normal vascular wall smooth muscle cells, especially in ruptured group. The density of vascular smooth muscle cells in the unruptured group was significantly lower than that in the normal control group (P0.01), and the density of vascular smooth muscle cells in the ruptured group was significantly lower than that in the unruptured group (P0.05). The expression level of TLR4NF- 魏 B CD68Caspase-3 in ruptured group and unruptured group was significantly higher than that in normal control group (P0.05). The relative expression of TLR4 and NF- 魏 B mRNA and protein in ruptured group and unruptured group were significantly higher than those in normal control group, and significantly higher in ruptured group than in unruptured group (P0.05). Conclusion TLR _ 4 / NF- 魏 B signaling pathway plays an important role in the pathogenesis of vascular wall inflammation and smooth muscle cell-mediated degenerative lesions in intracranial aneurysms induced by vascular endothelial injury.
【作者单位】：甘肃省人民医院西院区神经外科;
【分类号】：R743

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