补阳还五汤联合依达拉奉对小鼠脑缺血再灌注损伤的影响

发布时间：2018-08-11 21:26

【摘要】：目的研究补阳还五汤联合依达拉奉对小鼠脑缺血再灌注损伤后神经细胞凋亡及Bcl-2、Bax和Caspase-3表达的影响。方法 50只小鼠随机分假手术组,模型组,补阳还五汤(BYHWD)组,依达拉奉(ED)组,补阳还五汤+依达拉奉(BYHWD+ED)组,每组再随机分成缺血再灌注后1、7 d组。首先制备小鼠大脑中动脉缺血模型,TUNEL法观察神经细胞凋亡率,应用免疫组化方法观察各组大脑皮质Bcl-2、Bax、Caspase-3表达阳性细胞数。结果与模型组比较,BYHWD组、ED组及BYHWD+ED组均能明显降低脑组织凋亡指数(P0.01),升高Bcl-2阳性表达,降低Bax、Caspase-3阳性表达(P0.01),两药联用变化趋势更明显。结论补阳还五汤与依达拉奉联用能降低脑缺血再灌注损伤模型鼠脑组织细胞凋亡基因Bax、Caspase-3的表达,增加具有神经元保护作用的Bcl-2基因表达,从而抑制神经细胞凋亡,加速神经功能的恢复,协同发挥脑保护作用。
[Abstract]:Objective to study the effects of Buyang Huanwu decoction combined with Edaravone on neuronal apoptosis and expression of Bcl-2 Bax and Caspase-3 after cerebral ischemia reperfusion injury in mice. Methods Fifty mice were randomly divided into sham-operation group, model group, (BYHWD) group, (ED) group and (BYHWD ED) group. Each group was randomly divided into seven groups. The apoptosis rate of neurons was observed by Tunel method and the expression of Caspase-3 in the cerebral cortex of each group was observed by immunohistochemical method. Results compared with the model group, BYHWD group and BYHWD Ed group could significantly decrease the apoptosis index of brain tissue (P0.01), increase the positive expression of Bcl-2 and decrease the positive expression of Baxon Caspase-3 (P0.01). Conclusion the combination of Buyang Huanwu decoction and Edaravone can reduce the expression of apoptosis gene Baxfen Caspase-3 in brain tissue of cerebral ischemia reperfusion injury model, increase the expression of Bcl-2 gene with neuronal protection, and thus inhibit the apoptosis of nerve cells. To accelerate the recovery of neural function and to play a protective role in the brain.
【作者单位】：绍兴市人民医院浙江大学绍兴医院神经内科;
【基金】：绍兴市公益性技术应用研究计划项目(No.2013B70076)
【分类号】：R743

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