EGCG对小鼠实验性自身免疫性脑脊髓炎的保护作用

发布时间：2018-08-22 20:29

【摘要】：目的观察绿茶提取物EGCG对实验性自身免疫性脑脊髓炎(EAE)小鼠的治疗作用及相关机制。方法取C57BL/6小鼠,随机分为对照组、EAE模型组和EGCG治疗组,对各组小鼠进行神经功能评分,并对中枢神经系统炎性浸润情况进行观察;流式细胞技术检测Th1、Th17细胞在中枢神经系统中频率变化;免疫组化观察25 d脑组织IFN-γ、IL-17和TGF-β的表达情况;ELISA检测血清中IFN-γ、、IL-17、TGF-β、IL-10、IL-4的质量浓度。结果 EGCG治疗明显缓解EAE小鼠疾病症状和中枢神经系统炎症,抑制Th1与Th17细胞向中枢神经系统浸润。与EAE模型组相比,EGCG治疗组脑IFN-γ及IL-17表达上调,而TGF-β表达下调;外周血中可溶性上述细胞因子表达情况与脑一致。结论 EGCG可能通过影响中枢神经系统及外周血中IFN-γ、IL-17、TGF-β的表达,调控T细胞亚群的分化对EAE发挥保护作用。
[Abstract]:Objective to observe the therapeutic effect of green tea extract (EGCG) on experimental autoimmune encephalomyelitis (EAE) mice and its related mechanism. Methods C57BL/6 mice were randomly divided into two groups: the control group and the EGCG group. The neurological function of each group was evaluated and the inflammatory infiltration of the central nervous system was observed. The expression of IFN- 纬 IL-17 and TGF- 尾 in human brain tissue was detected by flow cytometry and the mass concentration of IL-10 IL-4 in serum was detected by Elisa. Results EGCG significantly alleviated the disease symptoms and central nervous system inflammation in EAE mice and inhibited the infiltration of Th1 and Th17 cells into the central nervous system. Compared with the EAE model group, the expression of IFN- 纬 and IL-17 was up-regulated, but the expression of TGF- 尾 was down-regulated, and the expression of soluble cytokines in peripheral blood was consistent with that in the brain. Conclusion EGCG may play a protective role on EAE by affecting the expression of IFN- 纬 IL-17 TGF- 尾 in the central nervous system and peripheral blood and regulating the differentiation of T cell subsets.
【作者单位】：江汉大学医学院免疫学教研室;江汉大学护理与医学技术学院;
【基金】：国家自然科学基金(81302528) 湖北省自然科学基金(2014CFB425) 武汉市科技计划(2015071704011601)
【分类号】：R744.51

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