人诱导多能干细胞用于亨廷顿病大鼠模型的移植治疗

发布时间：2018-08-28 13:46

【摘要】：目的探讨用于亨廷顿病(HD)治疗提供的药物筛选模型。方法 SD大鼠45只,分为正常对照组、毁损组、移植组三组,每组15只。正常组不予以处理,其余两组均用奎宁酸(QA)脑内注射,移植组在QA毁损模型建立后进行人诱导多能干细胞(hiPSC)移植。hiPSC在大鼠脑内存活了7 w,通过阿扑吗啡诱导旋转实验等行为学实验,观察各组大鼠运动功能情况;通过高十字迷宫、新环境抑制进食实验,观察各组焦虑类似的行为变化。结果与QA组相对比,观察到hiPSC移植组大鼠运动功能得到改善,大鼠焦虑类似的行为明显减少。hiPSC移植组大鼠的活动减少。结论hiPSC移植可以有效的改善HD大鼠的行为缺陷,hiPSC在治疗神经退行性疾病特别是HD,是一种理想的多潜能干细胞来源。
[Abstract]:Objective to investigate the drug screening model for (HD) treatment of Huntington's disease. Methods 45 SD rats were divided into normal control group, lesion group and transplantation group with 15 rats in each group. No treatment was given in the normal group, and the other two groups were injected with (QA). In the transplantation group, after the establishment of the model of QA damage, (hiPSC) transplantation of human induced pluripotent stem cells. HiPSC survived in the rat brain for 7 weeks. The motor function of each group was observed by behavioral experiments such as apomorphine induced rotation test, and the motor function was observed by high cross labyrinth. The new environment inhibited eating experiment and observed similar behavior changes of anxiety in each group. Results compared with the QA group, the motor function of the rats in the hiPSC transplantation group was improved, and the activity of the rats in the hiPSC transplantation group was significantly decreased. Conclusion hiPSC transplantation is an ideal source of multipotential stem cells for the treatment of neurodegenerative diseases, especially HD,.
【作者单位】：武汉市第三医院;武汉市武昌医院;
【基金】：湖北省自然科学基金资助项目(面上基金)(No.2014CFB450) 武汉市卫生计生委临床医学科研资助项目(No.wx15D21)
【分类号】：R-332;R742.2

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