丙戊酸钠对PD细胞模型的保护作用研究
[Abstract]:Parkinson's disease (Parkinson's disease, PD) is a common neurodegenerative disease with slow progression. So far, PD can not be completely cured. Sodium valproate (valproate, VPA), is used clinically to treat bipolar disorder and epilepsy, but also to relieve the symptoms of neurodegenerative diseases. Promote neuronal axon growth and inhibit neuronal apoptosis. Bundles and extension proteins (fasciculation andelongation protein zeta1, FEZ1) play an important role in the development of nerve cells, intracellular transport, apoptosis and release of neurotransmitters, and participate in the development of the central nervous system and the growth and bunching of axons. Adrenal pheochromocytoma (PC12,), which can synthesize and secrete dopamine, is a cell model of dopaminergic neurons cultured in vitro. It is often used to establish PD cell model in vitro. Aim: to study the effects of VPA on the proliferation, migration and FEZ1 expression of PC12 cells, and to establish a PD cell model to investigate the neuroprotective and antioxidant effects of VPA on 6-OHDA induced PC12 cell injury. Methods: (1) PC12 cells were treated with 0.5-0.75 mm VPA for 24 h. The morphological changes and the effects of cell migration were observed. The effect of VPA on cell proliferation was detected by MTT assay. Real-time PCR and Western blot were used to detect the expression of FEZ1. (2) the PD cell model was established, and the cells were divided into normal control group and 6-OHDA group. The dynamic changes of the cells were observed in the 6-OHDA group, and the proliferation of the cells in each group was detected by MTT method. Real-time PCR and Western blot were used to detect the expression of FEZ1 and apoptosis-related genes, and the activity of SOD and the content of MDA were detected. Results: (1) the results of MTT and cell scratch showed that VPA could promote the proliferation and migration of PC12 cells. The results of real-time PCR and Western blot showed that VPA could promote the expression of FEZ1mRNA and protein in a dose-dependent manner. In a dose-dependent manner. (2) the proliferation of PD cells was inhibited by PC12 cells treated with 6-OHDA, and then treated with 6-OHDA for 24 h after pretreatment with VPA for 24 h, the cell apoptosis was alleviated, the number of cell shrinkage was decreased, and the protrusions were elongated. The expression of FEZ1,Bcl-2 and Bax in each treatment group was detected by real-time PCR and Western blot. The results showed that the expression of FEZ1 and Bcl-2 decreased in the control group compared with the control group. The expression of FEZ1 and Bcl-2 in VPA 6-OHDA group was higher than that in 6-OHDA group. The results showed that the activity of SOD in VPA 6-OHDA group was lower than that in control group. The content of SOD in VPA 6-OHDA group was higher than that in 6-OHDA group, and the content of MDA in VPA 6-OHDA group was higher than that in 6-OHDA group. Conclusion: (1) VPA can promote the proliferation and migration of PC12 cells and increase the expression of FEZ1mRNA and its protein in a dose-dependent manner. (2) VPA can up-regulate the expression of FEZ1 and Bcl-2 in PD cell model induced by 6-OHDA and increase the level of anti-oxidation. It can reduce cell apoptosis, promote cell proliferation and play a neuroprotective role.
【学位授予单位】:苏州大学
【学位级别】:硕士
【学位授予年份】:2014
【分类号】:R742.5
【共引文献】
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