人参皂苷CK联合环磷酰胺对胶质瘤细胞的作用

发布时间：2018-09-01 12:49

【摘要】：目的观察人参皂苷CK联合烷化剂类化疗抗癌药物环磷酰胺对胶质瘤细胞的抑制作用。并探讨联合用药的剂量与胶质瘤细胞抑制率的关系。方法取对数生长期的胶质瘤细胞分组,分别为(1)环磷酰胺组;(2)人参皂苷CK组;(3)根据人参皂苷CK的抑瘤率结果,选择相应的浓度与环磷酰胺组成联合用药组。分别用不同浓度的环磷酰胺(30、35、40、45、50 ng/m L)、人参皂苷CK(10、15、20、25、30 mg/m L)和环磷酰胺(30、35、40、45、50 mg/m L)与人参皂苷CK(25、30 mg/m L)联合用药,与肿瘤细胞接触后,用MTT法进行活细胞数测定,观察药物对肿瘤抑制率的影响,并比较人参皂苷CK、环磷酰胺和人参皂苷CK联合环磷酰胺对胶质瘤细胞生长的敏感性。结果 MTT法检测显示人参皂苷CK对胶质瘤细胞有一定的抑制作用,并存在浓度依赖关系;当环磷酰胺浓度达到50 mg/m L时,对胶质瘤细胞的抑制率最大;人参皂苷CK联合环磷酰胺组对胶质瘤细胞的抑制率高于环磷酰胺组(P0.05)。结论人参皂苷CK联合环磷酰胺应用可使环磷酰胺在较低的浓度时抑制胶质瘤细胞的作用明显增强,人参皂苷CK起到了增敏协同的作用。
[Abstract]:Objective to observe the inhibitory effect of ginsenoside CK combined with anticancer agent cyclophosphamide on glioma cells. To investigate the relationship between the dosage of combined drug and the inhibition rate of glioma cells. Methods Glioma cells in logarithmic growth stage were divided into (1) cyclophosphamide group, (2) ginsenoside CK group, (3) the combination group composed of corresponding concentration and cyclophosphamide according to the results of inhibition rate of ginsenoside CK. Different concentrations of cyclophosphamide (30 ~ (35) ~ 40 ~ (455) ng/m L), ginsenoside CK (10 ~ 15 ~ 20 ~ 2530 mg/m / L) and cyclophosphamide (30 ~ 35 ~ 40 ~ (455) mg/m / L) and ginsenoside CK (25 ~ (30 mg/m / L) were used to determine the number of living cells after contact with tumor cells. To observe the effect of drugs on tumor inhibition rate and compare the sensitivity of ginsenoside CK, cyclophosphamide and ginsenoside CK combined with cyclophosphamide to glioma cell growth. Results MTT assay showed that ginsenoside CK could inhibit glioma cells in a concentration-dependent manner, and when the concentration of cyclophosphamide reached 50 mg/m / L, the inhibitory rate of ginsenoside CK on glioma cells was the highest. The inhibitory rate of ginsenoside CK combined with cyclophosphamide on glioma cells was higher than that of cyclophosphamide group (P0.05). Conclusion ginsenoside CK combined with cyclophosphamide can significantly enhance the inhibitory effect of cyclophosphamide on glioma cells at a lower concentration, and ginsenoside CK plays a synergistic role in enhancing the sensitivity of glioma cells.
【作者单位】：南方医科大学第三附属医院创伤救治中心;南方医科大学第三附属医院神经外科;
【分类号】：R739.4

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