自身免疫性脑炎患者外周血Tfh细胞的检测及其临床意义
发布时间：2018-09-01 16:13
【摘要】：目的:研究滤泡辅助性T细胞(Tfh)在自身免疫性脑炎(AE)患者外周血中的百分比及临床意义。方法:(1)采用自身免疫性脑炎相关抗体检测阳性的患者脑脊液与大鼠脑组织冰冻切片共孵育并进行免疫荧光染色,观察结果;(2)收集血清和/或脑脊液(CSF)检查自身免疫性脑炎相关抗体阳性的患者17例(其中,3例为抗LGI1抗体阳性患者,3例为抗GABAR抗体阳性患者,10例为抗NMDAR抗体阳性患者,1例为抗NMDAR抗体、抗GABAR抗体及抗AMPA2抗体合并阳性患者),收集外周血样本共计21例次:未治疗组6例次,治疗组15例次(其中,治疗后缓解组8例次,治疗后未缓解组7例次),健康对照者(对照组)外周血15例。采用流式细胞术(FCM)检测外周血单个核细胞(PBMC)中CD3+CD4+CXCR5+PD1+(即Tfh细胞)的比例,并分析Tfh细胞比例与患者疾病恢复程度的相关性。结果:(1)自身免疫性脑炎相关抗体检测阳性患者脑脊液与大鼠脑组织冰冻切片共孵育免疫荧光染色结果呈现明显阳性反应;(2)流式细胞术检测结果显示未治疗组患者外周血Tfh比例明显高于对照组(P0.001),且治疗未缓解组患者Tfh比例高于治疗缓解组(P0.05),而治疗缓解组患者Tfh比例与对照组比较,差异无统计学意义(P=0.107)。结论:自身免疫性脑炎患者外周血Tfh比例升高可能与疾病的发生发展相关。
[Abstract]:Objective: to study the percentage and clinical significance of follicular helper T cell (Tfh) in peripheral blood of patients with autoimmune encephalitis (AE). Methods: (1) the cerebrospinal fluid (CSF) of patients with autoimmune encephalitis related antibody was incubated with frozen sections of rat brain tissue and immunofluorescence staining was performed. (2) 17 cases of autoimmune encephalitis related antibody positive by (CSF) in serum and / or cerebrospinal fluid were collected (3 cases were positive for anti LGI1 antibody, 3 cases were positive for anti GABAR antibody, 10 cases were positive for anti NMDAR antibody) One patient with anti NMDAR antibody, The peripheral blood samples were collected for 21 times: 6 cases in the untreated group, 15 times in the treatment group (including 8 cases in the post-treatment remission group). After treatment, there were 7 cases in the unrelieved group and 15 cases in the healthy control group (the control group). The proportion of CD3 CD4 CXCR5 PD1 (Tfh cells) in peripheral blood mononuclear cells (PBMC) was detected by flow cytometry (FCM), and the correlation between the proportion of Tfh cells and the degree of disease recovery was analyzed. Results: (1) the results of immunofluorescence staining in cerebrospinal fluid (CSF) incubated with frozen sections of rat brain tissue were obviously positive, and (2) flow cytometry showed that the patients in untreated group showed positive reaction. The proportion of Tfh in peripheral blood was significantly higher than that in control group (P0.001), and the proportion of Tfh in non-remission group was higher than that in remission group (P0.05), while the proportion of Tfh in treatment group was higher than that in control group. The difference was not statistically significant (P = 0.107). Conclusion: the increased proportion of Tfh in peripheral blood of patients with autoimmune encephalitis may be related to the occurrence and development of the disease.
【作者单位】： 第四军医大学基础部免疫学教研室;第四军医大学西京医院神经内科;
【基金】：国家自然科学基金面上项目(No.81171977)资助
【分类号】：R742.9
，
本文编号：2217706
[Abstract]:Objective: to study the percentage and clinical significance of follicular helper T cell (Tfh) in peripheral blood of patients with autoimmune encephalitis (AE). Methods: (1) the cerebrospinal fluid (CSF) of patients with autoimmune encephalitis related antibody was incubated with frozen sections of rat brain tissue and immunofluorescence staining was performed. (2) 17 cases of autoimmune encephalitis related antibody positive by (CSF) in serum and / or cerebrospinal fluid were collected (3 cases were positive for anti LGI1 antibody, 3 cases were positive for anti GABAR antibody, 10 cases were positive for anti NMDAR antibody) One patient with anti NMDAR antibody, The peripheral blood samples were collected for 21 times: 6 cases in the untreated group, 15 times in the treatment group (including 8 cases in the post-treatment remission group). After treatment, there were 7 cases in the unrelieved group and 15 cases in the healthy control group (the control group). The proportion of CD3 CD4 CXCR5 PD1 (Tfh cells) in peripheral blood mononuclear cells (PBMC) was detected by flow cytometry (FCM), and the correlation between the proportion of Tfh cells and the degree of disease recovery was analyzed. Results: (1) the results of immunofluorescence staining in cerebrospinal fluid (CSF) incubated with frozen sections of rat brain tissue were obviously positive, and (2) flow cytometry showed that the patients in untreated group showed positive reaction. The proportion of Tfh in peripheral blood was significantly higher than that in control group (P0.001), and the proportion of Tfh in non-remission group was higher than that in remission group (P0.05), while the proportion of Tfh in treatment group was higher than that in control group. The difference was not statistically significant (P = 0.107). Conclusion: the increased proportion of Tfh in peripheral blood of patients with autoimmune encephalitis may be related to the occurrence and development of the disease.
【作者单位】： 第四军医大学基础部免疫学教研室;第四军医大学西京医院神经内科;
【基金】：国家自然科学基金面上项目(No.81171977)资助
【分类号】：R742.9
，
本文编号：2217706
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