左乙拉西坦对外伤性癫痫预防作用的实验研究

发布时间：2018-09-07 12:46

【摘要】：【目的】建立外伤性癫痫模型，观察不同剂量的左乙拉西坦预防性应用对大鼠行为学以及海马CA3区苔藓纤维出芽的影响，探讨左乙拉西坦对外伤性癫痫的预防作用。【方法】（1）外伤性癫痫模型建立：除生理盐水对照组外，其余大鼠均立体定向注射5μL浓度为0.2mol/L的氯化铁于左侧运动皮层，癫痫发作分级采用Racine（1972）评分标准，除死亡外发作至4级以及4级以上的随机分为5组，每组7只。（2）实验分组：A组：生理盐水对照组，等体积生理盐水连续灌胃7天；B组：模型对照组，左侧运动皮层注射氯化铁，而后给予等体积生理盐水连续灌胃7d；C组：75mg/kg剂量预防组，左侧运动皮层注射氯化铁，造模清醒后立即给予左乙拉西坦75mg/kg，1次/d，连续灌胃7d；D组：150mg/kg剂量预防组，左侧运动皮层注射氯化铁，造模清醒后立即给予左乙拉西坦150mg/kg，1次/d，连续灌胃7d；E组：300mg/kg剂量预防组，左侧运动皮层注射氯化铁，造模清醒后立即给予左乙拉西坦300mg/kg，1次/d，连续灌胃7d；F组：600mg/kg剂量预防组，左侧运动皮层注射氯化铁，造模清醒后立即给予左乙拉西坦600mg/kg，1次/d，，连续灌胃7d。（3）行为学观察：视频监控24h大鼠行为学变化，根据经典的癫痫发作行为评分标准对癫痫发作进行观测和记录[14]，记录大鼠造模后1w内癫痫发作次数。（4）海马苔藓纤维出芽Timm染色：大鼠造模后15d，海马冰冻切片做Timm染色，观察同侧海马CA3区苔藓纤维出芽情况，并选用Cavazos的标准对其进行评分。（5）统计学分析：结果用SPSS17.0统计软件进行单因素方差分析及LSD组间比较，评价左乙拉西坦对外伤性癫痫的预防作用。以P＜0.05为差别有统计学意义。【结果】（1）左乙拉西坦D、E、F组与模型对照组相比明显减少癫痫发作次数及海马CA3区苔藓纤维出芽程度，有统计学意义（P＜0.05），各预防组之间互相比较，除F组与E组之间差异无统计学意义以外（P0.05），其他各组之间，差异有统计学意义（P＜0.05）。（2）左乙拉西坦对减少癫痫发作次数以及抑制苔藓纤维出芽，效果与剂量呈正相关。【结论】左乙拉西坦在此模型中可以减少癫痫发作，抑制海马CA3区苔藓纤维出芽，提示左乙拉西坦有预防外伤性癫痫的作用。
[Abstract]:[objective] to establish a model of traumatic epilepsy and to observe the effects of different doses of levoethylacetam on the behavior of rats and the sprouting of mossy fibers in the CA3 area of hippocampus. To investigate the preventive effect of levoethylacetam on traumatic epilepsy. [methods] (1) the model of traumatic epilepsy was established: all rats except the control group were stereotactic injected with 5 渭 L 0.2mol/L ferric chloride in the left motor cortex. The epileptic seizures were divided into 5 groups (7 rats in each group) according to the Racine (1972) score. (2) the rats were divided into two groups: control group (n = 7): normal saline control group (n = 7), normal saline group (n = 7). Group B: the model control group, the left motor cortex was injected with ferric chloride, and then the same volume of normal saline was given continuously by stomach for 7 days. Group C was given the dose of 10% 75 mg / kg, the left motor cortex was injected with ferric chloride, and the left motor cortex was injected with ferric chloride. The rats were given levoethoxetam 75 mg / kg / kg once a day immediately after the model was made. The group D: 150 mg / kg was injected with ferric chloride for 7 days. The left motor cortex was injected with ferric chloride. Immediately after the model was made, the rats were given levoethoxetam 150 mg / kg / d for 7 days. Group E: 300 mg / kg, the left motor cortex was injected with ferric chloride, and immediately after the model was made, the rats were given levoethoxetam 300 mg / kg once a day, and the control group was given the dose of: 600 mg / kg for 7 days, and the left motor cortex was injected with ferric chloride at a dose of 600 mg / kg for 7 days, and the left motor cortex was injected with ferric chloride. Immediately after the model was made, the rats were given levoethoxetam 600 mg / kg / d for 7 days. (3) Behavioral observation: the behavioral changes of rats were monitored by video for 24 hours. The epileptic seizures were observed and recorded according to the classical scale of epileptic seizure behavior [14], and the number of epileptic seizures in the rat model was recorded within 1 week. (4) Timm staining of mossy fiber sprouting in hippocampus: the frozen sections of hippocampus were stained with Timm on the 15th day after the establishment of the model. The sprouting of mossy fibers in ipsilateral hippocampal CA3 region was observed, and the standard of Cavazos was selected to score it. (5) Statistical analysis: results univariate ANOVA and comparison between LSD groups were performed by SPSS17.0 statistical software. To evaluate the preventive effect of levoethylacetam on traumatic epilepsy. [results] (1) compared with the model control group, the number of epileptic seizures and the sprouting degree of mossy fiber in the CA3 region of the hippocampus were significantly decreased in the levoethoxetam group (P < 0. 05) and the control group (P < 0. 05). There was statistical significance (P < 0.05). There was no significant difference between group F and group E (P0.05). The difference was statistically significant (P < 0. 05). (2). The effect of levoethylacetam on reducing the number of seizures and inhibiting the sprouting of mossy fiber was positively correlated with the dosage. [conclusion] Levoethylacetam can reduce the seizure of epilepsy in this model. Inhibition of mossy fiber sprouting in hippocampal CA3 suggests that levoethylacetam can prevent traumatic epilepsy.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R742.1

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