酒精作用下小鼠海马区神经元放电规律研究
发布时间：2018-09-10 16:14
【摘要】：慢性和急性饮酒均可引起脑损伤,目前对急性饮酒导致的大脑记忆的损伤研究多采用生物化学及行为学方法。本文利用微电极阵列系统记录活体小鼠海马区神经元放电电位,分析并获得脑神经元放电规律,研究急性饮酒对小鼠记忆的影响。进一步通过激光散斑衬比成像实验和小鼠行为学T型迷宫实验,验证了神经元放电实验结果的正确性。论文主要研究内容包括:1、阐述了大脑神经元电信号与脑记忆生理特征的内在联系,以及神经元电生理技术在探讨急性饮酒对小鼠记忆影响方面的研究进展;2、编写了基于Matlab的信号预处理程序,实现了小鼠大脑海马CA1区的神经元放电信号的噪声滤除、锋电位特征提取与聚类;3、设计并完成了急性饮酒(重度、轻度)下小鼠的电生理实验,采集并分析了小鼠大脑CA1区锋电位的变化规律;另外,采用激光散斑衬比成像技术测量了脑局部血、利用T型迷宫研究了急性饮酒小鼠的方向选择正确率,验证了电生理实验结果;4、设计实验进一步研究了常用解酒药物葛根素对急性饮酒小鼠神经元放电规律的影响,研究了葛根素对大脑记忆的保护作用。论文采用多通道微电极阵列系统采集了急性饮酒小鼠大脑CA1区神经元锋电位,结果表明急性重度饮酒对小鼠海马区神经元放电具有抑制作用,急性轻度饮酒对海马区神经元放电具有促进作用。同时,葛根素对急性饮酒引起的神经元放电变化具有拮抗作用,证明了葛根素对饮酒机体大脑的保护作用。脑局部血流实验和行为学实验充分验证了电生理实验结果的正确性。论文的研究结论在探究急性饮酒对小鼠记忆的影响及葛根素对急性饮酒造成的大脑损伤的保护作用中具有重要的理论意义和临床应用价值。
[Abstract]:Both chronic and acute drinking can cause brain damage. At present, biochemical and behavioral methods are used to study the brain memory impairment caused by acute drinking. In this paper, the discharges of neurons in the hippocampal area of living mice were recorded by microelectrode array system, and the discharge patterns of brain neurons were analyzed and obtained, and the effects of acute drinking on the memory of mice were studied. The experimental results of firing of neurons were verified by laser speckle contrast imaging and behavioral T-maze test in mice. The main contents of this paper include: 1, the intrinsic relationship between the electrical signals of brain neurons and the physiological characteristics of brain memory. The research progress of neuron electrophysiology in the study of the effect of acute drinking on the memory of mice was also discussed. A signal preprocessing program based on Matlab was written to remove the noise of the neuron discharge signal in the CA1 region of the hippocampus of the mouse brain. The electrophysiological experiments of mice under acute drinking (severe and mild) were designed and completed. The changes of spike potentials in CA1 region of mice were collected and analyzed. The regional blood of brain was measured by laser speckle contrast imaging, and the correct rate of direction selection in acute drinking mice was studied by using T-maze. The experiment was designed to study the effect of puerarin, a common antialcoholic drug, on the firing of neurons in acute drinking mice and the protective effect of puerarin on brain memory. Multichannel microelectrode array system was used to collect neuronal spike potentials in CA1 area of acute drinking mice. The results showed that acute severe drinking could inhibit neuronal discharges in hippocampus of mice. Acute mild alcohol consumption can promote neuronal discharges in the hippocampal area. At the same time, puerarin has antagonistic effect on the changes of neuronal discharges induced by acute drinking, which proves the protective effect of puerarin on the brain of drinking body. The experimental results of local cerebral blood flow and behavior fully verify the correctness of the results of electrophysiological experiments. The conclusion of this paper has important theoretical significance and clinical application value in exploring the effect of acute drinking on the memory of mice and the protective effect of puerarin on brain injury caused by acute drinking.
【学位授予单位】：南京航空航天大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R741
本文编号：2234957
[Abstract]:Both chronic and acute drinking can cause brain damage. At present, biochemical and behavioral methods are used to study the brain memory impairment caused by acute drinking. In this paper, the discharges of neurons in the hippocampal area of living mice were recorded by microelectrode array system, and the discharge patterns of brain neurons were analyzed and obtained, and the effects of acute drinking on the memory of mice were studied. The experimental results of firing of neurons were verified by laser speckle contrast imaging and behavioral T-maze test in mice. The main contents of this paper include: 1, the intrinsic relationship between the electrical signals of brain neurons and the physiological characteristics of brain memory. The research progress of neuron electrophysiology in the study of the effect of acute drinking on the memory of mice was also discussed. A signal preprocessing program based on Matlab was written to remove the noise of the neuron discharge signal in the CA1 region of the hippocampus of the mouse brain. The electrophysiological experiments of mice under acute drinking (severe and mild) were designed and completed. The changes of spike potentials in CA1 region of mice were collected and analyzed. The regional blood of brain was measured by laser speckle contrast imaging, and the correct rate of direction selection in acute drinking mice was studied by using T-maze. The experiment was designed to study the effect of puerarin, a common antialcoholic drug, on the firing of neurons in acute drinking mice and the protective effect of puerarin on brain memory. Multichannel microelectrode array system was used to collect neuronal spike potentials in CA1 area of acute drinking mice. The results showed that acute severe drinking could inhibit neuronal discharges in hippocampus of mice. Acute mild alcohol consumption can promote neuronal discharges in the hippocampal area. At the same time, puerarin has antagonistic effect on the changes of neuronal discharges induced by acute drinking, which proves the protective effect of puerarin on the brain of drinking body. The experimental results of local cerebral blood flow and behavior fully verify the correctness of the results of electrophysiological experiments. The conclusion of this paper has important theoretical significance and clinical application value in exploring the effect of acute drinking on the memory of mice and the protective effect of puerarin on brain injury caused by acute drinking.
【学位授予单位】：南京航空航天大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R741
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