颈交感神经阻滞对糖尿病大鼠脑线粒体功能的影响

发布时间：2018-09-12 09:43

【摘要】：目的观察颈交感神经阻滞对糖尿病大鼠脑线粒体功能的影响。方法雄性成年大鼠40只随机分为3组:对照组(n=10,腹腔注射注射等量缓冲液),糖尿病组(n=15,采用STZ糖尿病模型按65 mg/kg剂量腹腔1次注射1%STZ制备糖尿病大鼠),糖尿病+颈交感神经阻滞组(n=15,按65 mg/kg剂量腹腔1次注射1%STZ,糖尿病成模后8周给予颈交感神经阻滞治疗)。造模后12周水迷宫实验观察各组大鼠的认知功能。并于12周处死大鼠,采用Clark氧电极法测定大鼠海马线粒体的呼吸功能,免疫组化检测海马IL-6、TNF-α的表达。结果造模后12周,糖尿病组存活12只,阻滞组存活13只。糖尿病组、阻滞组与对照组相比,逃避潜伏期明显延长、穿越平台的次数明显减少(P0.05)。糖尿病组线粒体呼吸功能为R3(28.51±3.01)、R4(13.93±1.16)、RCR(2.09±0.27),阻滞组的为R3(45.26±4.21)、R4(11.99±1.11)、RCR(3.52±0.32),对照组为R3(66.31±5.65)、R4(12.97±3.38)、RCR(4.78±0.68),糖尿病组和阻滞组线粒体呼吸功能均较对照组组明显下降,主要表现为R3和RCR值显著降低(P0.05);但阻滞组的R3和RCR高于糖尿病组(P0.05)。在海马CA1区,糖尿病组IL-6、TNF-α表达为(53.32±6.29)、(63.21±13.24),阻滞组为(28.14±3.82)、(32.76±5.98),均高于对照组(13.87±2.54)、(11.09±8.87)(P0.05)。阻滞组明显低于糖尿病组,差异有统计学意义(P0.05)。结论颈交感神经阻滞可改善糖尿病大鼠脑线粒体功能,其机制可能与其下调IL-6、TNF-α有关。
[Abstract]:Objective to observe the effect of cervical sympathetic block on mitochondrial function in diabetic rats. Methods Forty adult male rats were randomly divided into three groups: control group (n = 10, intraperitoneal injection of the same amount of buffer), diabetic group (n = 15, STZ diabetes model was injected intraperitoneally with 1%STZ at a dose of 65 mg/kg), diabetes mellitus (DM). Cervical sympathetic nerve block group (NN15, 65 mg/kg intraperitoneal injection of 1 STZ, diabetes model 8 weeks after the treatment of cervical sympathetic nerve block). The cognitive function of rats in each group was observed by water maze experiment 12 weeks after modeling. The respiratory function of hippocampus mitochondria was measured by Clark oxygen electrode method and the expression of IL-6,TNF- 伪 in hippocampus was detected by immunohistochemistry. Results 12 weeks after modeling, 12 rats survived in diabetic group and 13 survived in block group. Compared with the control group, the escape latency was significantly prolonged and the frequency of crossing the platform was significantly decreased in the diabetic group and the block group (P0.05). R3 (28.51 卤3.01) R4 (13.93 卤1.16) RCR (2.09 卤0.27), R3 (45.26 卤4.21) R4 (11.99 卤1.11) RCR (3.52 卤0.32), R3 (66.31 卤5.65) R4 (12.97 卤3.38) RCR and block group (4.78 卤0.68) in diabetic group and block group respectively. R3 and RCR decreased significantly (P0.05), but R3 and RCR in block group were higher than that in diabetic group (P0.05). In hippocampal CA1 area, the expression of IL-6,TNF- 伪 in diabetic group (53.32 卤6.29), (63.21 卤13.24) and block group (28.14 卤3.82), (32.76 卤5.98) was higher than that in control group (13.87 卤2.54), (11.09 卤8.87) (P0.05). Block group was significantly lower than diabetes group, the difference was statistically significant (P0.05). Conclusion Cervical sympathetic nerve block can improve mitochondrial function in diabetic rats, and its mechanism may be related to its down-regulation of IL-6,TNF- 伪.
【作者单位】：贵州省人民医院疼痛科;贵州省人民医院神经内科;
【基金】：贵州省社会发展科技攻关项目(黔科合SY字[2013]3037号) 贵州省科技厅基金(黔科合J字[2013]2184)~~
【分类号】：R587.2;R747.9

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