肢体缺血后处理对大鼠慢性脑缺血后脑内LINGO-1的表达及神经功能的变化

发布时间：2018-09-18 16:17

【摘要】：目的:观察大鼠肢体缺血后处理后脑内LINGO-1表达的变化,研究肢体缺血后处理改善慢性脑缺血后神经损伤的分子机制。方法:采用50%三氯化铁处理双侧颈总动脉的方法建立慢性脑缺血模型,随机将大鼠分为假手术组、模型组和肢体缺血后处理组。大鼠肢体缺血后处理3周后采用三等分Y型电迷宫方法观察各组大鼠的学习记忆能力,RT-qPCR检测LINGO-1的mRNA,Western blot法测定LINGO-1的蛋白水平。结果:与模型组相比,肢体缺血后处理组大鼠学习记忆能力显著增加(P0.05)。假手术组大鼠LINGO-1的mRNA及蛋白水平有少量阳性表达。肢体缺血后处理组大鼠LINGO-1的mRNA及蛋白水平均显著下降(P0.05)。结论:慢性脑缺血大鼠脑内LINGO-1水平明显上调,肢体缺血后处理可明显降低LINGO-1的表达水平,并提高学习记忆能力,这可能是肢体缺血后处理改善慢性脑缺血神经功能的分子机制之一。
[Abstract]:Aim: to observe the changes of LINGO-1 expression in the brain of rats after limb ischemia and to study the molecular mechanism of improving the nerve injury after limb ischemia. Methods: the model of chronic cerebral ischemia was established by 50% ferric chloride in bilateral common carotid artery. Rats were randomly divided into sham-operation group, model group and limb ischemic post-treatment group. After limb ischemia for 3 weeks, the learning and memory ability of rats in each group was observed by three equal Y-type electrical maze method. The protein level of LINGO-1 was detected by RT-qPCR with mRNA,Western blot method. Results: compared with the model group, the ability of learning and memory was significantly increased in the post-ischemic limb group (P0.05). There was a little positive expression of mRNA and protein in LINGO-1 in sham operation group. The levels of mRNA and protein in LINGO-1 of rats after limb ischemia were significantly decreased (P 0.05). Conclusion: the level of LINGO-1 in the brain of rats with chronic cerebral ischemia can be upregulated obviously, and the expression of LINGO-1 and the ability of learning and memory can be significantly decreased after limb ischemia. This may be one of the molecular mechanisms to improve the neural function of chronic cerebral ischemia after limb ischemia.
【作者单位】：济南大学山东省医学科学院医学与生命科学学院;山东省医学科学院药物研究所国家卫生部生物技术药物重点实验室山东省罕少见病重点实验室;平阴县中医医院;
【基金】：山东省重大新药创制中心建设项目(No.2009ZX09301-013)
【分类号】：R743

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