舌静脉注射没食子酸的慢性低灌注大鼠学习记忆能力及脑氧化应激损伤相关指标观察

发布时间：2018-09-18 19:04

【摘要】：目的观察舌静脉注射没食子酸(GA)的慢性低灌注大鼠学习记忆能力及脑氧化应激损伤相关指标变化。方法 48只大鼠随机分为4组各12只。A、B组大鼠制备慢性低灌注模型,A组在制模前后各5天舌静脉注射100 mg/kg GA,B组给予等量生理盐水;C、D组参照A组手术方法处理大鼠但不结扎双侧颈总动脉,C组在手术前后各5天舌静脉注射100 mg/kg GA,D组给予等量生理盐水。比较各组大鼠的学习记忆能力(逃避潜伏期、目标象限所用时间百分比、游泳速度)及脑氧化应激损伤相关指标[超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)、总硫醇(TT)]。结果与D组比较,B组第1~4天逃避潜伏期增加(P均0.05);与B组比较,A组第1~4天逃避潜伏期减少(P均0.05)。与D组比较,B组目标象限所用时间百分比减少(P均0.05);与B组比较,A、C组目标象限所用时间百分比增加(P均0.05)。与D组比较,B组SOD、GSH-Px、TT降低,MDA升高(P均0.05);与B组比较,A组和C组SOD、GSH-Px、TT升高,MDA降低(P均0.05)。结论 GA能改善慢性低灌注大鼠的学习记忆能力及修复脑氧化应激损伤。
[Abstract]:Objective to observe the changes of learning and memory ability and brain oxidative stress injury in chronic hypoperfusion rats with oral Gallic acid (GA) injection. Methods Forty-eight rats were randomly divided into 4 groups (n = 12 each) and 12 rats in group A (n = 12). But the group C without ligation of bilateral common carotid artery was given the same amount of normal saline 5 days before and after operation. The learning and memory abilities (escape latency, percentage of time used in target quadrant, swimming speed) and the related indexes of brain oxidative stress injury [superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA), total mercaptan (TT)] were compared in each group. Results compared with group D, the escape latency of group B was significantly higher than that of group D (P 0.05), and that of group A was significantly lower than that of group B (P 0.05). Compared with group D, the percentage of time used in target quadrant in group B was decreased (P 0.05), and the percentage of time used in target quadrant in group C was higher than that in group B (P 0.05). Compared with group D, SOD,GSH-Px,TT in group B was lower than that in group D (P 0.05), and SOD,GSH-Px,TT in group A and group C was higher than that in group B (P 0.05). Conclusion GA can improve the ability of learning and memory and repair the oxidative stress injury in chronic hypoperfusion rats.
【作者单位】：中国医科大学附属盛京医院;
【分类号】：R743

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